Optimization of purification process of Guava polyphenols with macroporous absorbent resin

To find a better method to separate and purify polyphenols in Guava, the absorption capability and purification effect of polyphenols of Guava on macroporous absorption resin were evaluated.Adsorption and desorption experiments were carried out to screen the suitable macroporous absorbent resin for Guava polyphenols.Results showed that NKA-9 was the best macroporous resin for purification.The optimum absorption condition of macroporous resin NKA-9 were concertration of feeding 1.2mg/mL, pH2.0, feeding rate 1mL/min.Under this condition, the absorption ratio was 90.5%, the desorption conditions were ethanol concentration 50% pH3.0, elute rate 1mL/min.Under this condition, the desorption ratio was 89.3%.