Study on construction of yeast engineering strain for pear wine by protoplast fusion method

The yeast engineering strain was structured by the protoplast fusion technology.Saccharomyces cerevisiae YDJ05 and Issatchenkia orientalis YS03 were used as the fusion parents X and Y.A arginine nutrition unfairness strain was selected by EMS mutagenesis from parents strains Y.The strains X and Y were dealt with the snail enzyme liquid in 35℃ for 100min.The best protoplast formation rate and regeneration rate were 93.6%, 94.2% and 27.8%, 31.6%.Inactivate strains of the parents of X with heating them in 14min.In the circumstance with PEG as promotion melting agent DJ01 ~ DJ06 six strains fusant were gotten, one of them DJ02 with the best alcohol producting rate and aroma producting rate were reached to 9.87% and 0.37g/L.