Optimization of polysaccharide extraction technology from Glycyrrhiza inflata Bat by response surface methodology

Polysaccharide extracted from Xinjiang Glycyrrhiza inflate Batalin were studied using ethanol as the extraction solvent. Based on the single factor test analysis, the effects of three variables (namely extraction temperature extraction time, and liquid-solid ratio) on the glycyrrhizia polysaccharide (GPS) extraction efficiency and the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. The results indicated that extraction temperature of 93℃, extraction time of 83min, and liquid-solid ratio of 21∶ 1 (mg∶ L) were the optimum condition. Under these optimized conditions, the experimental values of GPS yield was 10. 48%, in good agreement with the predictive value 10. 59%. Therefore, the GPS extraction from Glycyrrhiza inflata Batalin optimized by response surface methodology was reliable.