The establishment of C57/BL6 mice as a model of shrimp allergy

In order to establish the C57/BL6 mouse allergic model and in vitro cell model of its mast cell, six-week -old C57/BL6 mice were immunized with shrimp protein extracts. Western Blot was used to analyze the specificity of sera IgE and IgG1 from the immunized mice. Sensitized peritoneal mast cells (PMC) were collected and induced to release histamine with various kinds of shrimp allergens, and then histamine was detected by HPLC and fluorescence microplate reader, respectively. Western Blot showed tropomyosin at 36ku could be recognized by 57. 1% IgE and 74. 1% IgG1 form immunized mice sera, 80ku shrimp allergen by 42. 9% IgG1 and IgE, and 21ku shrimp allergen by 28. 6% IgG1 and 42. 9 % IgE. The release rates of histamine detected by HPLC and fluorescence microplate reader with no significant difference, and the highest histamine release rate with 21. 59% and 18. 52% from PMC induced by tropomyosin, followed by 80ku and 21ku allergens. Tropomyosin was the major shrimp allergen of C57/BL6 mice, and 80ku and 21ku allergens were the minor allergens, which was identical with human shrimp allergy. The results demonstrated that C57/BL6 mouse was a very ideal shrimp allergic animal model and PMC was an available cell model to detect and assess food allergens.