Detection and analysis of antibiotic resistance genes and integron of Escherichia coli isolated from fish pond ecological constellation by mutiplex PCR

Integrase and integron in E. coli could provide reliable scientific foundation to the control of the spread of antibiotic resistance of bacteria. In this study, assessed the situation of antibiotic resistance genes (ARG) , 165 strains of E. coli isolated from ecological constellation were screened for 19 antibiotic resistance genes using multiplex PCR assays and it was found that 91. 51% of E. coli carried ARGs. The results showed that it was popular to carry ARGs in E. coli strains. Among them, E. coli strains carried multiplex ARGs played a dominant role, and there were about 86. 06% of E. coli strains carried more than 2 ARGs. Then multiplex PCR assay was used to detect the strains carried more than 4 ARGs for integrase genes. The PCR result revealed that the frequency of class I integrase gene was 87. 84%, the proportion of class II integrase gene was 10. 81%. None of class III integrase gene was found in the test. Moreover, the results indicated that integron-cassette system predominated in the antibiotic resistant machanism mediated by integron.