Meat species authenticity identification by loop-mediated isothermal amplification assay in beef and mutton

A loop-mediated isothermal amplification (Lamp) method was developed for meat ingredients authenticity identification in beef and mutton. A set of universal primer used for Lamp at 63℃ was chosen to differentiate animal gene encoding cytochrome b with high sensitivity. 0.01%～2% pork could be detected in beef and mutton by the method. Besides, the method could be used to detect animal meats processed or unprocessed.