Optimizing enzymatic hydrolysis conditions of sheep bone protein through quadratic orthogonal rotation combination design

The fresh sheep bone was hydrolyzed with alcalase and flavourzyme.The optimization of hydrolysis conditions of sheep bone protein was carried out and mathematical model was established through the quadratic regression orthogonal rotary combination design.The results showed that when the initial pH8.5 and temperature was 55℃, the optimal conditions were alkaline protease dosage of 3000u/g, the role of alkaline protease time was 5h, flavourzyme addition level was 3500u/g, the role of alkaline protease time was 3.5h, the substrate concentration was 10%.