High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans

Resting cells of G.oxydans DSM2003 is used to produce the 2-keto-gluco-nate, which is a primary precursor for the synthesis of D-erythoribic acid (EA) .One bacterium with high-yield production capacity was obtained by self-adapting to the high concentration of substrate, and the conversion rate had reached 24.68mmol/L/h in flask (temperature at 30℃, pH at 6.0, cell concentration at 20g/L, substrate concentration at 1100mmol/L, shaking rate at 250r/min) .While catalysis was carried in the 7L fermentor under the optimum condition, the conversion rate of 2-KGA could reach 74mmol/L/h and the cell could be reused effectively for three times.