Isolation and identification of a kind of dominant bacteria and its variation from puer tea in the fermentation process

A kind of dominant bacteria was found from the fermentation heap of Menghai Tea industry in 2010.According to the bacteria for separation and purification, the bacteria was observed by morphological characteristics and microscopic structure in a variety of medium and confirmed DNA sequencing of tested strains, it was identified as Aspergillus niger.And variation of the bacteria in the fermentation process was studied on fermentation with the heap of tests.Results showed that the number of Aspergillus niger was up to maximum at the first seven day in the same fermentation cycle (magnitude to 107CFU/g) , the late gradually reduced;with the extension of time of fermentation environment, its number was greater;at the different seasons of fermentation process, the trend of quantitative of Aspergillus niger was summer>autumn>spring>winter.