Optimization of Extraction Process and Component Analysis of Anthocyanins from Grape Pomace in Aqueous Two-phase Extraction with Genetic Algorithm
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摘要: 以葡萄皮渣为原料，采用双水相法提取其中的花色苷并鉴定花色苷组分。在单因素实验的基础上，通过遗传算法优化双水相法提取葡萄皮渣花色苷的工艺；利用高效液相色谱-质谱联用鉴定葡萄皮渣花色苷提取物中花色苷组分。结果表明：双水相法提取葡萄皮渣花色苷的最佳工艺为：乙醇体积分数40%、硫酸铵质量分数26%、pH3.0、料液比1：38 g/mL，在此条件下花色苷得率（3.05±0.07） mg/g。经鉴定发现葡萄皮渣花色苷提取物含有2种花色苷组分，分别为飞燕草-3-葡萄糖苷和矢车菊素-3-葡萄糖苷，其纯度分别为90.16%和92.41%。研究结果为天然花色苷提取提供一种新的提取方式，并为进一步开发花色苷功能性食品提供依据。Abstract: The anthocyanins were extracted from grape pomace by aqueous two-phase extraction. Genetic algorithm was employed to optimize the extraction process of anthocyanins from grape pomace based on single factor experiment. HPLC-MS was used to identify the anthocyanins components of the extracts from grape pomace obtained under the optimal combination of process parameters. The optimum extracting parameters to achieve the highest the yield of anthocyanins of(3.05±0.07) mg/g from grape pomace by aqueous two-phase method was obtained under an ethanol concentration of 40%,ammonium sulfate mass fraction of 26%,pH of 3.0,solid-to-liquid ratio of 1:38 g/mL. Under these conditions,the yield of anthocyanims was(3.05±0.07) mg/g.The anthocyanins extracts from grape pomace mainly contained two anthocyanins components in terms of delphinium-3-glucoside and cyanidin-3-glucoside,and their purities were 90.16% and 92.41%,respectively. The results of natural anthocyanins extraction would provide a new way and basis for further development of functional food.
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Keywords:
- aqueous two-phase extraction /
- grape pomace /
- anthocyanins /
- process /
- components
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