Effects of Chimonanthus salicifolius Aleoholic Extracts on Growth, Muscle Quality and Intestinal Morphology of Larimichthys crocea
-
摘要: 目的：研究柳叶蜡梅醇提物对大黄鱼生长性能、肌肉品质和肠道形态的影响。方法：选取体质健康、平均体质量为10.31±2.88 g的大黄鱼500尾。各组分别在基础饲料中添加浓度为0、0.5%、1.0%、2.0%、4.0%的柳叶蜡梅醇提物，设为L-0（设为对照组）、L-0.5、L-1、L-2、L-4组。试验周期为8周。试验考察了大黄鱼的生长性能，肌肉质构、pH、系水力、肌纤维结构及肠道形态等指标。结果：在生长性能方面，与L-0相比，L-0.5、L-1、L-2、L-4组均能够显著提高大黄鱼的存活率（P<0.05）；柳叶蜡梅醇提物能改善大黄鱼肌肉品质和肌纤维结构。其中L-4效果最好，与L-0相比，其肌肉硬度、胶粘性、内聚力、咀嚼性和肌纤维密度均显著提高（P<0.05），失水率、冷冻渗出率、pH下降率、肌纤维直径则显著降低（P<0.05）；在肠道形态方面，与L-0相比，L-2、L-4组均显著提高了肠道绒毛长度和绒毛长度与隐窝深度之比（P<0.05），并降低了隐窝深度（P<0.05）；相关性分析表明，柳叶蜡梅醇提物的浓度与大黄鱼肌纤维直径呈极显著（P<0.01）负相关关系；与鱼肠道绒毛长度呈极显著（P<0.01）正相关关系。同时，进一步分析发现大黄鱼肌纤维直径又与肌肉硬度、内聚力、胶粘性和咀嚼性呈显著（P<0.05）负相关关系；且内聚力与绒毛长度呈显著（P<0.05）正相关关系。结论：添加4%柳叶蜡梅醇提物的饲料能改善大黄鱼肌肉品质和肠道形态，其机理可能是由于柳叶蜡梅醇提物改善了大黄鱼消化道形态，促进营养物质吸收的同时，改善肌纤维形态，进而促进肌肉品质的改善。Abstract: Objective:To study the effects of Chimonanthus salicifolius aleoholic extracts on growth,muscle quality and intesinal morphology of Larimichthys crocea.The growth performance,muscle texture,pH,water holding capacity,muscle quality and intestinal morphology of Larimichthys crocea were investigated. Methods:A total of 500 health Larimichthys crocea with an body weight about 10.31±2.88 g were randomly divided into 5 groups. The fish in the 5 groups were fed with the basal diets supplemented Chimonanthus salicifolius aleoholic extract at the concentrations of 0(control group),0.5%(L-0.5),1.0%(L-1),2.0%(L-2),4.0%(L-4),respectively. The experiment lasted for 8 weeks. Results:Compared with the control group,the survival rate of Larimichthys crocea in each experimental group was significantly improved(P<0.05). Chimonanthus salicifolius aleoholic extracts improved the muscle quality and muscle fiber structure of Larimichthys crocea. Compared with the control group,L-4 group significantly increased the muscle hardness,adhesiveness,cohesion,mastication and muscle fiber density(P<0.05)and significantly reduced the fiber diameter(P<0.05). L-2 and L-4 groups significantly increased intestinal villi length and the ratio of the villi length to the crypt depth(P<0.05)and decreased the crypt depth(P<0.05)compared with the control group. In the correlation analysis,the concentration of Chimonanthus salicifolius aleoholic extracts was negatively correlated with muscle fiber diameter and positively correlated with villus length of Larimichthys crocea. Hardness,cohesion,adhesiveness,and mastication were negatively correlated with muscle fiber diameter. Cohesion was positively correlated with villus length. Conclusion:The muscle quality and intestinal morphology of Larimichthys crocea could be improved by adding the 4% aleoholic extract into fodder. The mechanism were related to Chimonanthus salicifolius aleoholic extracts improving the digestive tract morphology,promoting the absorption of nutrients,improving the muscle fiber morphology and promoting the improvement of muscle quality of Larimichthys crocea.
-
Keywords:
- Chimonanthus salicifolius /
- growth /
- muscle quality /
- intestinal morphology
-
[1] 胡云华. 2017福建宁德大黄鱼市场调查报告[J]. 广东饲料,2018,27(4):13-15. [2] 张瑞霞,张娟,熊善柏,等. 低温处理对鲫生化特性及肉质的影响[J]. 华中农业大学学报,2008(4):532-535. [3] 李松,郭全友,李保国,等. 养殖模式和饵料对养殖大黄鱼体色、质构和营养成分评价的影响[J]. 食品与发酵工业,2019,45(21):118-125. [4] 谭娟,邓雨飞,曹宇舰,等. 饲料中添加复方中草药对草鱼幼鱼生长、肌肉成分及免疫相关酶活性的影响[J]. 广东农业科学,2015,42(10):109-113. [5] 曲木,黄成,张宝龙,等. 复方中草药对黄颡鱼生长、肉质及血清中补体C3、补体C4含量的影响[J]. 中国饲料,2018,(19):74-79. [6] Omitoyin B O,Ajani E K,Orisasona O,et al. Effect of guava Psidium guajava(L.)aqueous extract diet on growth performance,intestinal morphology,immune response and survival of Oreochromis niloticus challenged with Aeromonas hydrophila[J]. Aquaculture Research,2019,50(7):1851-1861.
[7] 王伟影,毛菊华,王发英,等. 基于一测多评法研究畲药食凉茶黄酮类物质动态变化规律[J]. 中草药,2017,48(12):2532-2537. [8] Wang N,Chen H,Xiong L,et al. Phytochemical profile of ethanolic extracts of Chimonanthus salicifolius S. Y. Hu. leaves and its antimicrobial and antibiotic-mediating activity[J]. Industrial Crops & Products,2018,125:328-334.
[9] Ciftci O,Ozdemir I,Tanyildizi S,et al. Antioxidative effects of curcumin,beta-myrcene and 1,8-cineole against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rats liver[J]. Toxicol Ind Health,2011,27(5):447-453.
[10] Choi E J,Ahn W S. Kaempferol induced the apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 cells[J].Nutr Res Pract,2008,2(4):322-325.
[11] 耿敬章. 柳叶腊梅叶总黄酮超声波协同复合酶提取及抗氧化活性研究[J]. 食品工业科技,2016,37(21):124-129. [12] 邹永,范振鑫,魏日凤,等. 柳叶蜡梅醇提物对无水乙醇致大鼠急性胃黏膜损伤的保护作用[J]. 食品工业科技,2020,41(18):294-300. [13] Tan X H,Sun Z Z,Huang Z,et al. Effects of dietary hawthorn extract on growth performance,immune responses,growth-and immune-related genes expression of juvenile golden pompano(Trachinotus ovatus)and its susceptibility to Vibrio harveyi infection[J]. Fish & Shellfish Immunology,2017,70:656-664.
[14] 黄光中,胡辉,罗世民,等. 葡萄籽等植物提取物对黄鳝促生长机制的研究[J]. 当代水产,2012,37(9):76-79. [15] 李栋. 竹取物在食品品质改良及风险控制中的应用基础研究[D].杭州:浙江大学,2012. [16] 吴素玲,孙晓明,金敬红,等. 乙醇渗漉法提取青花椒生物碱的工艺参数[J]. 中国野生植物资源,2015,34(5):1-3. [17] 杜松云,杨艾玲,毛淑芳. 优选咳喘灵凝胶膏剂的渗漉法提取工艺[J]. 广东化工,2016,43(4):21-22. [18] 吴金平,杜浩,陈细华,等. 烟酸对杂交鲟幼鱼生长性能、肌肉品质和抗氧化功能的影响[J]. 中国水产科学,2020,27(4):406-413. [19] 谢苏明,王裕玉,聂志娟,等. 投喂频率对池塘工程化循环水养殖大口黑鲈生长、生理及肝脏GH、IGF-I基因表达丰度的影响[J]. 中国水产科学,2020,27(4):363-374. [20] 许晓莹. 杜仲、蚕豆饲料对草鱼生长、肌肉品质和胶原蛋白基因表达的影响[D].上海:上海海洋大学,2018. [21] Fuentes A,Fernández-Segovia I,Serra J A,et al. Comparison of wild and cultured sea bass(Dicentrarchus labrax)quality[J]. Food Chemistry,2010,119(4):1514-1518.
[22] Periago M J,Ayala M D,López-Albors O,et al. Muscle cellularity and flesh quality of wild and farmed sea bass,Dicentrarchus labrax L.[J]. Aquaculture,2005,249(1):175-188.
[23] Johnston I A,Alderson R,Sandham C,et al. Muscle fibre density in relation to the colour and texture of smoked Atlantic salmon(Salmo salar L.)[J]. Aquaculture,2000,189(3):335-349.
[24] Picha M E,Turano M J,Beckman B R,et al. Endocrine biomarkers of growth and applications to aquaculture:A minireview of growth hormone,insulin-like growth factor(IGF)-I,and IGF-binding proteins as potential growth indicators in fish[J].North American Journal of Aquaculture,2008,70(2):196-211.
[25] 邓超准. 养殖密度和原花青素对星洲红鱼生长及生理生化的影响[D].厦门:集美大学,2015. [26] 杨继华,冰陈,黄燕华,等. 饲料中添加桑叶黄酮对吉富罗非鱼生长性能、体成分、抗氧化指标及抗亚硝酸盐应激能力的影响[J]. 动物营养学报,2017,29(9):3403-3412. [27] 周茜,杨建乔,董小涵,等. 复方中草药对肉鸡生长性能、肌肉品质、免疫功能和肠道菌群的影响[J]. 食品工业科技,2017,38(17):84-87. [28] 何丽娜. 发酵工艺对鲤鱼质构及食用品质的影响[D].无锡:江南大学,2019. [29] Srikanchai T,Srikanchai T,Murani E,et al. Four loci differentially expressed in muscle tissue depending on water-holding capacity are associated with meat quality in commercial pig herds[J]. Molecular Biology Reports,2010,37(1):595-601.
[30] Savage A W J,Warriss P D,Jolley P D. The amount and composition of the proteins in drip from stored pig meat[J]. Meat Science,1990,27(4):289-303.
[31] Luciano G,Monahan F J,Vasta V,et al. Dietary tannins improve lamb meat colour stability[J]. Meat Science,2009,81(1):120-125.
[32] 吴姝,蒋步云,宋泽和,等. 植物多酚对黄羽肉鸡抗氧化性能、肠道形态及肉品质的影响[J]. 动物营养学报,2018,30(12):5118-5126. [33] 缪函霖,包海蓉,赵路漫. 金枪鱼肉冷藏过程中理化特性的变化[J]. 江苏农业科学,2013,41(8):248-251. [34] 王汉玲,刘彩华,秦军委,等. 羟自由基氧化系统对草鱼肌肉蛋白理化特性及其保水性的影响[J]. 食品科技,2018,43(1):134-140. [35] 吴娟. 一水肌酸对肉鸭胴体组成、肉品质及宰后肌肉能量代谢的影响研究[D].南京:南京农业大学,2011. [36] Hurling R,Rodell J B,Hunt H D. Fiber diameter and fish texture[J]. Journal of Texture Studies,1996,27(6):679-685.
[37] 关磊,朱瑞俊,李小勤,等. 普通草鱼与脆化草鱼的肌肉特性比较[J]. 上海海洋大学学报,2011,20(5):748-753. [38] 尹丽卿,苏琳,靳烨. 肌纤维特性与鱼肉品质的关系及其调控技术的研究进展[J]. 食品工业,2015,36(2):231-234. [39] He X,Lu Z,Ma B,et al. Effects of chronic heat exposure on growth performance,intestinal epithelial histology,appetite-related hormones and genes expression in broilers[J]. J Sci Food Agric,2018,98(12):4471-4478.
[40] 温爽,郭宇荣,杨澜,等. 腹水综合征肉鸡空肠黏膜组织结构及相关基因表达分析[J]. 中国畜牧兽医,2019,46(6):1825-1831. [41] 王巍,甘佳,付茂忠,等. 日粮能量和蛋白质水平对蜀宣花牛肉品质的影响[J]. 黑龙江畜牧兽医,2020(7):54-57. [42] 关文静,朱艺峰,陈芝丹. 鱼类肌纤维特性与鱼肉品质关系[J]. 水产科学,2008(2):101-104. [43] 贾艳华,杨宪时,许钟,等. 水分含量对软烤扇贝质构和色泽的影响[J]. 食品与机械,2010,26(3):47-50.
计量
- 文章访问数: 209
- HTML全文浏览量: 23
- PDF下载量: 11
下载:
