Regulation of High Content of TG-DHA Deodorized Fish Oil on Lipid Metabolism
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摘要: 目的：探究TG-DHA高含量脱腥鱼油的制备及对高脂饮食小鼠脂代谢的调节作用。方法：脂质体包埋技术制备TG-DHA高含量脱腥鱼油。将雄性C57BL/6J小鼠（6周龄）随机分为对照组（C）、模型组（M）、TG-DHA高含量鱼油组（O-DHA）、TG-DHA高含量脱腥鱼油组（L-DHA）。连续灌胃8周后，检测小鼠血清、肝脏脂质水平；测定肝脏脂代谢相关基因脂肪酸合成酶（Fatty acid synthase，FAS）、固醇调节元件结合蛋白1c （Sterol regulatory element binding protein-1c，SREBP-1c）、肉碱棕榈酰转移酶1（Carnitine palmitoyl transferase，CPT1）、脂蛋白脂酶（Lipoprotein lipase，LPL） mRNA表达量。结果：脂质体包埋掩盖了己醛、2，4-庚二烯醛等鱼油中的腥味物质。TG-DHA高含量脱腥鱼油中的DHA含量为73.74%，粒径为159.50 nm，Zeta电位为-43.10 mV，稳定性良好，且在动物体内易于被消化吸收。L-DHA可显著改善高脂饮食小鼠血清和肝脏脂质水平（P<0.05），O-DHA可显著改善高脂饮食小鼠血清水平和肝脏中的TG含量（P<0.05），二者均能极显著降低FAS mRNA表达（P<0.01），显著上调CPT1 mRNA表达（P<0.05），且L-DHA效果更好。结论：TG-DHA高含量脱腥鱼油可改善高脂饮食导致的脂代谢紊乱，其机制与下调肝脏脂肪酸合成，促进脂肪酸分解代谢有关。Abstract: Objective:To investigate the effect of deodorized Triglyceride type-Docosahexaenoic acid(TG-DHA)fish oil on lipid metabolism in mice fed with high-fat diet. Methods:The deodorized TG-DHA fish oil was prepared by liposome embedding technology. Male C57BL/6J mice(6 weeks old)were housed and randomly divided into control group(C),model group(M),TG-DHA fish oil group(O-DHA),deodorized TG-DHA fish oil group(L-DHA).After 8 weeks,blood and liver were collected for lipid accumulation analysis. For gene expression mRNA was extracted from liver for fatty acid synthase(FAS),sterol regulatory element binding protein 1c(SREBP 1c),carnitine palmityl transferase 1(CPt1),lipoprotein lipase(LPL). Results:Liposome embedding could mask the fishy odour in fish oil such as hexanal,2,4-heptadienal. The content of DHA was 73.74%,the particle size was 159.50 nm,and the zeta potential was-43.10 mV. The liposome was stable and easy to be digested and absorbed in animals. L-DHA significantly improved the serum and hepatic lipid levels in mice fed with a high-fat diet(P<0.05),O-DHA significantly improved the serum level and liver TG content of mice fed with high-fat diet(P<0.05). Both of them significantly decreased FAS mRNA expression(P<0.01),and significantly increased CPT1 mRNA expression(P<0.05). The effects of L-DHA seemed to be better. Conclusion:Deodorized TG-DHA fish oil can improve lipid metabolism by high-fat diet. The mechanism is related to the down-regulation of liver fatty acid synthesis and the promotion of fatty acid catabolism.
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Keywords:
- TG-DHA /
- liposomes /
- deodorized fish oil /
- high-fat diet /
- lipid metabolism
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