摘要:
采用超声辅助提取野金柴中的黄酮类化合物,研究液料比、乙醇浓度、超声功率、超声时间对提取得率的影响,并采用响应曲面法优化提取工艺条件。采用ADS-7大孔树脂对野金柴中的根皮苷进行分离,分析总黄酮、根皮苷、黄酮R(除去根皮苷后的剩余黄酮组分)的抗氧化活性。结果表明,各工艺条件对野金柴总黄酮得率的影响为:超声时间>乙醇浓度>超声功率>液料比,优化所得最佳的提取工艺条件为:液料比40∶1,乙醇浓度80%,超声功率540 W,超声时间60 min,在此条件下野金柴总黄酮得率为8.82%±0.09%。总黄酮、根皮苷、黄酮R清除DPPH自由基的IC50值分别为0.0205、0.0222、0.0261 mg/mL;清除ABTS自由基的IC50值分别为0.0220、0.0233、0.0266 mg/mL,总黄酮抗氧化能力最强,根皮苷其次,再次为黄酮R,三者都有较好的DPPH和ABTS自由基清除能力。
关键词:
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响应面法
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超声提取
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野金柴
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黄酮
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抗氧化
Abstract:
The flavonoids of Lithocarpus polystachyus Rehd were extracted by ultrasound assisted method. The effects of liquid-material ratio,ethanol concentration,ultrasonic power,and ultrasonic time on the extraction yield were studied,and the response surface methodology was used to optimize the extraction process conditions. ADS-7 macroporous resin was utilized to separate the phlorizin,and the antioxidant activities of total flavonoids,phlorizin,and flavonoid R(the remaining flavones after phlorizin removal)were analyzed. The results indicated that the order of factors affecting the total flavonoid extraction yield was the ultrasonic time>ethanol concentration>ultrasonic power>liquid-material ratio. The optimized parameters were as follows:Liquid-material ratio 40∶1,ethanol concentration 80%,ultrasound power of 540 W,and ultrasound time of 60 min. Under these conditions,the total flavonoid yield was 8.82%±0.09%. The IC50 values of DPPH free radical scavenging by total flavonoids,phlorizin,and flavonoids R were 0.0205,0.0222,and 0.0261 mg/mL,respectively. The IC50 values of total flavonoids,phlorizin,and flavonoids R scavenging ABTS free radicals were 0.0220,0.0233,and 0.0266 mg/mL. The antioxidant capacity of total flavonoids was the strongest,followed by phlorizin and then by flavonoid R,all of which showed great DPPH and ABTS free radical scavenging ability.
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