Optimization of Extraction and Identification of Potassium Hydroxycitrate from Fruits of Garcinia cambogia L.
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摘要: 为了从藤黄果果实中制备游离羟基柠檬酸钾盐,采用水提取法对藤黄果果实粉末进行提取,通过单因素实验和正交试验对提取工艺进行优化,并对提取物进行了偏矾酸铵显色、薄层层析和HPLC鉴定,得到最佳条件为:料液比为1:18、浓缩倍数为5倍、酒精度数为60%vol、pH为12。各因素对游离羟基柠檬酸回收率效果影响大小依次为料液比>浓缩倍数>pH>酒精度数。在该最佳条件下,藤黄果羟基柠檬酸钾的得率为3.24%±0.32%。该方法在提取过程中采用KOH在偏碱性条件下保护游离羟基柠檬酸,形成羟基柠檬酸盐,经HPLC鉴定为与羟基柠檬酸标品保留时间相同的单峰,纯度为52.10%±0.53%,优于现有工艺水平。Abstract: In order to prepare the potassium hydroxycitrate from the fruits of Garcinia cambogia L.,the water extraction method was adopted to extract hydroxycitric acid from the powders prepared from G. cambogia L. fruits. The extractions were identified by the chromogenic reaction with ammonium monovanadate,thin layer chromatographic analysis and the HPLC analysis. The extraction process was optimized by the single factor test and the orthogonal test. The optimal conditions were as follows:The solid liquid ratio was 1:18,the condensive fold was 5,the alcohol degree was 60%vol,and the pH value was 12. The effects of various factors on the recovery rate of free hydroxycitric acid were in the order of solid liquid ratio>condensive fold>pH>alcohol degree. Under these optimal conditions,the recovery rate of potassium hydroxycitrate from the fruits of G. cambogia L. was 3.24%±0.32%. In the extraction process,KOH was used to protect the free hydroxycitric acid under the alkaline condition to form the potassium hydroxycitrate. A single peak whose retention time was the same to that of the hydroxycitric acid was detected by HPLC and its purity was 52.10%±0.53%. This method was better than the existing technology.
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Keywords:
- Garcinia cambogia L. /
- hydroxycitric acid /
- extraction /
- alkaline condition /
- purity
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