摘要:
目的:研究苦荞麸皮总黄酮(Total Flavonoids from Tartary Buckwheat Bran,TFTB)的体外抗氧化活性及体内解酒护肝作用。方法:在体外抗氧化活性实验中,观察TFTB对超氧阴离子自由基(O2-·)、DPPH·及NO2-离子的清除效果。在解酒护肝实验中,将50只小鼠随机分为模型组、阳性药对照组(150 mg/kg·bw)、TFTB低、中、高剂量组(100、200、300 mg/kg·bw)。各组小鼠一次性灌胃溶媒或药物30 min后,再一次性灌胃38% vol浓香型白酒(0.20 mL/10 g),测定小鼠醉酒率、醉酒耐受时间、醉睡时间,以及血清中谷草转氨酶(Aspartate Aminotransferase,AST)和肝脏中丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)水平。结果:TFTB对超氧阴离子自由基(O2-·)、DPPH·及NO2-离子均有一定的清除作用,其半抑制浓度(IC50)分别为1.36、0.02、1.79 mg/mL。与醉酒模型组对比,TFTB高剂量组醉酒耐受时间显著延长(P<0.05),TFTB各组醉睡时间极显著缩短(P<0.01);血清中AST含量显著降低(P<0.05),肝组织中SOD活性极显著提高(P<0.01),MDA含量显著降低(P<0.05)。结论:TFTB表现出较强的体外抗氧化活性,具有一定的防醉解酒作用,且在一定程度上能缓解小鼠的酒精性肝损伤。
关键词:
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黄酮
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苦荞麸皮
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抗氧化活性
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醉酒
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保肝作用
Abstract:
Objective:To study the antioxidant activity in vitro,and relieving alcoholism and hepatoprotective effect in mice by total flavonoids from tartary buckwheat bran(TFTB). Method:In vitro antioxidant experiments,the scavenging effects of TFTB on superoxide anion radical(O2-·),DPPH·and NO2- were observed. In the alcohol-relieving and hepatoprotective experiments,fifty mice were randomly divided into drunkenness model group,positive control group(150 mg/kg·bw),TBTF low,medium and high dose group(100,200 and 300 mg/kg·bw). After a one-time gavage of vehicle or drug for 30 min,the mice in each group were given a one-time gavage of Luzhou-flavor liquor of 38%vol(0.20 mL/10 g),and then the drunkenness rate,tolerance time of drunkenness,time of drunkenness and the levels of aspartate aminotransferase(AST)in serum,malondialdehyde(MDA)and superoxide dismutase(SOD)in liver were determined. Results:TFTB had a certain scavenging effect on superoxide anion radical(O2-·),DPPH· and NO2-,and the inhibitory concentration 50%(IC50)values were 1.36,0.02 and 1.79 mg/mL. Compared with the model group of drunkenness,the tolerance time of drunkenness in the high dose group of TFTB was significantly prolonged(P<0.05),the groups of TFTB also had a shortened drunkenness(P<0.01),a significant decrease of AST level in serum(P<0.05),and a significant increase SOD activity(P<0.01)and a significant decrease of MDA level in liver(P<0.05). Conclusion:TFTB shows strong antioxidant activity in vitro,and has a certain anti-alcoholic effect and can alleviate alcoholic liver injury in mice to a certain extent.
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