Screening of Broad-Spectrum Antibacterial Paenibacillus polymyxa and Physicochemical Properties of Its Bacteriocin
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摘要: 采用热处理和琼脂扩散法从湖泥中筛选出一株产广谱抗菌活性物质的芽孢杆菌XW4,通过形态观察、生理生化实验、16S rDNA分析等对其进行鉴定,并对其发酵产物进行抑菌活性探究。结果表明XW4在细菌发育分类学上鉴定为多粘类芽孢杆菌,其发酵产物对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌、赖氨酸芽孢杆菌等多种腐败微生物均有抑制作用XW4。XW4无细胞上清液对蛋白酶k敏感,对木瓜蛋白酶、胰蛋白酶、胃蛋白酶不敏感;经60、80、100℃处理30 min后,对大肠杆菌拮抗活性基本不变;在pH3.0~11.0范围内保持其抑菌活性。Abstract: One strain of Bacillus named XW4 was isolated from lake mud with a broad spectrum of antimicrobial activity by heat treatment and agar diffusion method. Bacillus XW4 was identified by its morphological and cultural characteristics,physiological and biochemical properties and 16S rDNA sequence analysis. And the antibacterial activity of its fermentation products was also determined. The results showed that the strain XW4 was identified as Paenibacillus polymyxa,and its secondary metabolites had strong antimicrobial activities against some kinds of spoilage microorganisms,including Bacillus subtilis,Staphyloccocus aureus Escherichia coli and Lysinibacillus fusiformis. The cell-free supernatants from Bacillus was sensitive to proteinase k,while without sensitive to papain,trypsin and pepsin. The antimicrobial activity to Escherichia coli was stable after heat treatment with 60,80,100 ℃,and it maintained effective within pH3.0~11.0.
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Keywords:
- broad antibacterial activity /
- Paenibacillus polymyxa /
- bacteriocin /
- identification
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