摘要:
以陕产瞿麦挥发油得率作评价指标,在单因素实验基础上,应用响应曲面法,优选出了微波辅助提取陕产瞿麦中挥发油的最佳工艺,并对其抗氧化性进行研究。结果表明,微波辅助提取挥发油的最佳工艺为:微波功率500 W,液料比20:1 mL/g,提取温度46℃,在此条件下得到的挥发油得率达3.19%±0.46%,与模型预测值3.27%基本相符,说明该模型合理可靠。体外抗氧化试验表明:微波法提取的挥发油和同等条件下超声法(功率120 W)提取的挥发油对DPPH·和O2-·清除能力呈较好的量效关系。微波法和超声法提取的挥发油在实验浓度范围内对DPPH·自由基清除的IC50值分别为0.82、0.73 mg/mL,对超氧阴离子自由基清除的IC50值分别为0.68、0.81 mg/mL,说明陕产瞿麦中挥发油具有一定的抗氧化活性,不同的提取方法得到的挥发油对不同自由基的清除能力略有不同。
Abstract:
Based on single factor experiments,the yield of volatile oil as an evaluation index,the best technology of microwave-assisted extraction of volatile oil from Shaanxi-fringed pink was determined with response surface method,and the antioxidant activity of the extracted volatile oil was studied. Results showed that,the optimal conditions of microwave-assisted extraction were:Microwave power was 500 W,liquid-solid ratio was 20:1 mL/g,extraction temperature was 46 ℃. Under these conditions,the yield of volatile oil was 3.19%±0.46%,which was in line with the model predicted value 3.27%. The model was reasonable and reliable. The antioxidant tests in vitro showed that the volatile oil extracted by microwave method and the volatile oil extracted by ultrasonic method(power 120 W)under the same conditions had a good dose-effect relationship for DPPH· and O2-· scavenging capacity. Within the experimental concentration range,the IC50 values of DPPH· free radical scavenging of volatile oil extracted by microwave and ultrasonic methods were 0.82 and 0.73 mg/mL respectively,and the IC50 values of superoxide anion free radical scavenging were 0.68 and 0.81 mg/mL respectively.The results indicated that the volatile oil from Shaanxi-fringed pink had certain antioxidant activity,and the scavenging abilities of the volatile oil obtained by different extraction methods were slightly different for different free radicals.
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