Determination of α-Solanine and α-Chaconine in Potato and Potato Powder by High Performance Liquid Chromatography Tandem Mass Spectrometry
-
摘要: 建立了高效液相色谱-串联质谱同时测定马铃薯及马铃薯粉中α-茄碱与α-卡茄碱含量的方法。样品采用10%乙酸水溶液匀浆提取3 min,马铃薯粉提取液经Bond Elut C18 SPE柱净化后,采用C18色谱柱分离,液相色谱串联质谱仪检测。结果表明,α-茄碱和α-卡茄碱在0.01~1.00 μg/mL范围内线性关系良好,相关系数分别为0.9998和0.9997。马铃薯和马铃薯粉在低、中、高三个添加浓度范围内,回收率在90.0%~110%之间,相对标准偏差在1.6%~5.0%之间。马铃薯中α-茄碱和α-卡茄碱的定量限分别为0.80和0.42 μg/kg,马铃薯粉中α-茄碱和α-卡茄碱的定量限分别为25和14 μg/kg(S/N=10)。本方法简单快速,灵敏度高,重复性好,适用于马铃薯及马铃薯粉中的α-茄碱和α-卡茄碱检测。
-
关键词:
- 高效液相色谱串联质谱 /
- 马铃薯 /
- α-茄碱 /
- α-卡茄碱
Abstract: A high performance liquid chromatography-tandem mass spectrometry method(HPLC-MS/MS)was established to detect α-solanine and α-chaconine in potato and potato powder samples simultaneously. The samples were extracted with 10% acetic acid aqueous solution for 3 min,and potato powder samples were purified with Bond Elut C18 SPE column. The extraction was analysed by HPLC-MS/MS with C18 chromatographic column. The results showed that correlation coefficients were 0.9998 for α-solanine and 0.9997 for α-chaconine ranged from 0.01 μg/mL to 1.00 μg/mL. The recoveries of spiked potato and potato powder samples were 90%~110% with the relative standard deviation(RSD)from 1.6% to 5.0% in low,medium and high concentration. The limit of quantity(LOQ)of α-solanine and α-chaconine in potato samples was 0.80 μg/kg and 0.42 μg/kg respectively(S/N=10),and the LOQ in potato powder samples was 25 μg/kg and 14 μg/kg respectively(S/N=10). The method was simple and rapid with high sensitivity and repeatability,and it was suitable for simultaneous detection of α-solanine and α-chaconine in potatoes and potato powder samples.
计量
- 文章访问数: 296
- HTML全文浏览量: 60
- PDF下载量: 28
下载:
