金刚烷胺残留化学发光酶免疫法的建立

崔廷婷; 冯才伟; 吴小胜; 王兆芹; 焦强; 李静; 张颖; 万宇平

doi:10.13386/j.issn1002-0306.2019.14.045

金刚烷胺残留化学发光酶免疫法的建立

崔廷婷^1,2,
冯才伟^1,2,
吴小胜^1,2,
王兆芹^1,2,
焦强³,
李静^1,2,
张颖^1,2,
万宇平^1,2, ,

1. 北京勤邦生物技术有限公司, 北京 102206;
2. 北京市食品安全免疫快速检测工程技术研究中心, 北京 102206;
3. 河南省口岸食品检验检测所, 河南郑州 450003

基金项目:

科技新星与领军人才培养计划项目(Z171100001117158)。

详细信息

作者简介:
崔廷婷(1985-),女,硕士,高级工程师,研究方向:食品分析,E-mail:ctt0312@163.com。

通讯作者:
万宇平(1982-),男,本科,高级工程师,研究方向:食品安全快速检测,E-mail:beijingqinbang@163.com。

中图分类号: TS201.2
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出版历程
- 收稿日期: 2018-10-15
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-07-14

Establishment of Chemiluminescent Enzyme Immunoassay for Determination of Amantadine Residues

1. Beijing Kwinbon Biotechnology Co., Ltd., Beijing 102206, China;
2. Beijing Engineering Research Centre of Food Safety Immunodetection, Beijing 102206, China;
3. Food Inspection and Testing Institute of Henan Province, Zhengzhou 450003, China

摘要

摘要: 建立金刚烷胺残留的化学发光酶免疫方法。对酶标抗原与磁标抗体的最佳稀释倍数、反应时间、底物发光时间进行优化,建立标准曲线,同时对方法的检测限、准确度和精密度进行评价。结果表明:50%抑制浓度(IC₅₀)为0.481 μg/L,线性范围是0.085~2.729 μg/L;在动物组织、兽药、饲料中的检测限为0.1 μg/kg,样本添加回收率为80.2%~94.5%,批内、批间相对标准偏差均<10%。该方法灵敏度高、操作简便,可广泛用于动物组织、兽药、饲料中金刚烷胺残留量的测定。
- 金刚烷胺 /
- 化学发光酶免疫法 /
- 动物组织 /
- 兽药 /
- 饲料
Abstract: The aim of the study is to establish a method for determination of amantadine residues by chemiluminescent enzyme immunoassay (CLEIA). After optimizing the dilution multiple of enzyme-labeled antigen and magnetic labeled antibody, reaction time and substrate luminescence time, a standard curve was established, and finally the detection limit, accuracy and precision of the method were evaluated. The results showed that the 50% inhibitory concentration (IC₅₀) was 0.481 μg/L, and the linearity range was 0.085~2.729 μg/L. The detection limit in animal tissues, veterinary drugs and feed were 0.1 μg/kg. The recoveries ranged from 80.2%~94.5%, with the intra-assay and inter-assay coefficients of variation less than 10%. The method is sensitive, simple and it can be widely used for the determination of amantadine residues in animal tissues, veterinary drugs and feed.
- amantadine /
- chemiluminescent enzyme immunoassay /
- animal tissues /
- veterinary drugs /
- feed