Screen and Optimization of the Methods for Removing Protein from Crude Polysaccharide in Ginkgo Biloba Leaves
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摘要: 本文以除蛋白率和多糖损失率为指标,比较了Sevage法、TCA法、等电点法和酶法清除银杏落叶粗多糖中蛋白的效果,筛选出最优除蛋白方法,并寻求银杏落叶粗多糖除蛋白的最佳工艺。结果表明,酶法和等电点法都为除蛋白较好方法,并优化酶-等电点结合法除蛋白的最佳工艺条件为:酶添加量为0.90%(以底物计算),酶解时间为1.1 h,酶解温度46 ℃,酶解pH为4.7。在此条件下,除蛋白率92.73%±0.13%,多糖损失率9.28%±0.12%。此方法除蛋白率较高,多糖损失率较低,为多糖在食品领域中的利用提供实验依据。Abstract: In this paper,the effects of sevage method,TCA method,isoelectric point method and enzymatic method on the removal of ginkgo biloba leaves polysaccharide protein were compared with the indicator of the protein clearance and loss rate of polysaccharide.Results showed that both enzymatic and isoelectric methods were good methods for removing proteins. And the best optimized steps of enzymatic-isoelectric point method were as follows:The enzyme addition amount of 0.90%(calculated as substrate),the enzymatic hydrolysis time of 1.1 h,the enzymatic hydrolysis temperature of 46 ℃,and the enzymatic hydrolysis pH of 4.7. Under the conditions,the protein loss rate was 92.73%±0.13%,and the polysaccharide loss rate was 9.28%±0.12%. This method had a higher protein rate and loss rate of polysaccharide was low. It would provide an experimental basis for the utilization of polysaccharides in the food field.
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