超声波辅助酶法提取葡萄皮渣多酚工艺优化

陈瑞喜; 王璐璐; 陈德蓉; 雷芳萍; 杨学山; 祝霞

doi:10.13386/j.issn1002-0306.2019.09.034

超声波辅助酶法提取葡萄皮渣多酚工艺优化

陈瑞喜¹,
王璐璐¹,
陈德蓉¹,
雷芳萍¹,
杨学山^1,2,
祝霞^1,2, ,

1. 甘肃农业大学食品科学与工程学院, 甘肃兰州 730070;
2. 甘肃省葡萄与葡萄酒工程学重点实验室, 甘肃兰州 730070

基金项目:

国家自然基金地区基金项目(31660455

甘肃省重点研发计划(17YF1NA060)。

31760454)

甘肃农业大学SRTP重点项目(20180946)

详细信息

作者简介:
陈瑞喜(1997-),男,大学本科,研究方向:葡萄与葡萄酒,E-mail:2741425119@qq.com;王璐璐(1992-),女,硕士研究生,研究方向:葡萄与葡萄酒,E-mail:2544621439@qq.com。

通讯作者:
祝霞(1977-),女,硕士,副教授,研究方向:葡萄酒风味品质调控,E-mail:zhux@gsau.edu.cn。

中图分类号: TS255.1
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出版历程
- 收稿日期: 2018-08-13
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-04-30

Process Optimization for Ultrasonic-Assisted Enzymatic Extraction of Polyphenols from Grape Pomace

1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
2. Gansu Key Laboratory of Viticulture and Enology, Lanzhou 730070, China

摘要

摘要: 以赤霞珠葡萄皮渣为原料,采用超声波辅助酶法提取多酚化合物。在探讨超声时间、超声功率、酶解pH及酶解温度对葡萄皮渣多酚得率影响的基础上,应用正交试验优化超声波辅助酶法提取葡萄皮渣多酚的工艺条件。结果表明:影响多酚得率的主次因素为超声时间＞酶解温度＞超声功率＞酶解pH,最佳工艺参数为超声时间15 min,超声功率400 W,酶解pH6.0,酶解温度60 ℃,在此工艺条件下多酚得率最高为1.493%±0.0068%。该工艺成本低、简单快速、稳定可行且提取剂环保无公害,因此可以有效替代传统方法提取葡萄皮渣中的多酚。
- 葡萄皮渣 /
- 多酚 /
- 超声波 /
- 纤维素酶
Abstract: Taking Cabernet Sauvignon grape pomace as the research material,polyphenols were extracted by ultrasonic-assisted enzymatic method. Based on the influence of ultrasonic treatment time,ultrasonic power,enzymatic hydrolysis pH and enzymatic hydrolysis temperature on the yied of grape marc polyphenols were discussed,the optimal conditions of ultrasonic-assisted enzymatic extraction of polyphenols from grape pomace were determined using the orthogonal experiments. The results showed that the primary and secondary factors affecting polyphenols yield were ultrasonic time > enzymatic hydrolysis temperature > ultrasonic power > enzymatic hydrolysis pH. The optimal process parameters identified were as follows:ultrasonic time was 15 minutes,ultrasonic power was 400 W,enzymatic hydrolysis pH was 6.0,enzymatic hydrolysis temperature was 60 ℃,under these process conditions,the highest polyphenol yield could reach 1.493%±0.0068%. The process is less expensive,simple and ripe,stable and feasible,and since it is based on the environmentally friendly extraction solvent,it may provide a valuable alternative to the conventional extraction methods.
- grape pomace /
- polyphenols /
- ultrasonic /
- cellulose