甜樱桃采后腐烂病原菌的分离与鉴定

王楚; 张倩; 李阳; 牛宇; 杨埔; 牛伟; 张丽珍

doi:10.13386/j.issn1002-0306.2019.02.028

甜樱桃采后腐烂病原菌的分离与鉴定

王楚¹,
张倩¹,
李阳¹,
牛宇²,
杨埔¹,
牛伟³,
张丽珍^1, ,

1. 山西大学生命科学学院, 山西太原 030006;
2. 山西省农业科学院, 农业资源与经济研究所, 山西太原 030006;
3. 山西省农业科学院, 山西太原 030031

基金项目:

山西省科技厅重点研发计划(农业)(201603D221014,201803D221008-9)。

详细信息

作者简介:
王楚(1990-),女,硕士研究生,研究方向:果蔬保鲜,E-mail:15835107995@163.com。

通讯作者:
张丽珍(1977-),女,博士,教授,研究方向:生物质资源筛选及应用,E-mail:lizhen@sxu.edu.cn。

中图分类号: TS255.1
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出版历程
- 收稿日期: 2018-06-24
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-01-14

Isolation and Identification of Postharvest Pathogens of Sweet Cherry

1. School of Life Science, Shanxi University, Taiyuan 030006, China;
2. Institute of Agricultural Resources and Economy, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China;
3. Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China

摘要

摘要: 为了研究山西省甜樱桃果实采后贮藏过程中的病原菌,本文对低温贮藏过程中发生腐烂的甜樱桃果实的病原菌进行分离纯化。在形态学基础上,结合基因序列分析,对分离获得的2株病原真菌YTA、YTB进行鉴定。YTA和YTB利用真菌通用引物ITS序列进行PCR扩增,将测序所得的基因序列与NCBI的GenBank进行同源序列比对,确定甜樱桃病原菌的生物学分类。结果表明:病原菌YTA为草酸青霉(Penicillium oxalicum),YTB为葡萄孢属(Botrytis sp.),将其重新回接到甜樱桃上,均能引起甜樱桃腐烂。草酸青霉(Penicillium oxalicum),葡萄孢属(Botrytis sp.)均是造成山西省甜樱桃采后腐烂的病原菌。
- 甜樱桃 /
- 病原真菌 /
- 分离 /
- 鉴定
Abstract: In order to investigate the pathogens of sweet cherry fruit during storage in Shanxi province, the pathogens from rottensweet cherry fruit during cryopreservation were isolated and purified.On the basis of morphology, combined with gene sequence analysis, the two pathogenic fungi YTA and YTB were identified.The ITS sequences of pathogenic fungi YTA and YTB were obtained by PCR using universal primers for fungi. The sequence of the sequenced gene was aligned with the NCBI GenBank to determine the biological classification of the sweet cherry pathogen.The results showed that YTA was Penicillium oxalicum, YTB was Botrytis sp., and sweet cherry inoculated with the two strains were decayed and showed the samesymptom, compared with naturally decayed sweet cherry. In summary, Penicillium oxalicum and Botrytis sp. were the pathogens causing postharvest decay of sweet cherry in Shanxi Province.
- sweet cherries /
- pathogen fungi /
- screening /
- identification