摘要:
为了比较不同萃取方法对茯砖茶挥发性成分的萃取效果,找到更适合茯砖茶挥发性成分分析的萃取方法,采用气相色谱-三重四极杆串联质谱(Gas Chromatography-triple Quadrupole Series Mass Spectrometry,GC-QQQ-MS)比较分析了同时蒸馏萃取法(Simultaneous Distillation Extraction,SDE)和顶空固相微萃取法(Head Space Solid-phase Micro-extraction,HS-SPME)提取茯砖茶挥发性成分的效果。结果表明:两种方法提取到的茯砖茶挥发性成分差异明显,两种方法共提取出90种挥发性成分,共有成分40种。其中SDE法提取到79种,以碳氢化合物、杂氧化合物、酮类和酚类为主;主要化合物有植酮、雪松醇、棕榈酸、2,6-二叔丁基对甲酚、二苯并呋喃、菲、香叶基丙酮、邻苯二甲醚、邻甲酚、联苯等。HS-SPME法提取到50种,以碳氢化合物、杂氧化合物、酮类和酚类为主;主要化合物有咖啡因、邻苯二甲酸二乙酯、二苯并呋喃、二氢猕猴桃内酯、联苯、香叶基丙酮、植酮、雪松醇、菲、1,2,3-三甲氧基苯等。两种方法各有优劣,相互补充,结合分析可为茯砖茶挥发性成分鉴定提供更全面、更完整、更准确的信息。
Abstract:
In order to compare the extraction efficiencies of volatile compounds by different methods from Fu brick Tea,find an extraction method which was more suitable for the analysis of volatile components in Fu brick Tea. Gas chromatography-triple quadrupole series mass spectrometry(GC-QQQ-MS)was used to analysis of volatile components from Fu brick Tea. Compared with the effects of simultaneous distillation extraction(SDE)and head space solid-phase micro-extraction(HS-SPME)on the aroma components of Fu brick tea and the conditions of head space solid-phase micro-extractions were optimized. The results showed that the volatile components of Fu brick Tea from different extract method showed large differences. There were 90 kinds of volatile components combined extraction by the two methods,with 40 kinds of common components. Among them,79 kinds of compounds were extracted by SDE,mainly consisting of hydrocarbons,oxygen heterocyclic compounds,ketones and alcohols. The main compounds were phytone,cedrol,palmitic acid,butylated hydroxytoluene,dibenzofuran,phenanthrene,6,10-dimethyl-5,9-undecadien-2-one,1,2-dimethoxy-benzene,2-methyl-phenol,biphenyl. 50 kinds of compounds were extracted by HS-SPME,mainly consisting of hydrocarbons,oxygen heterocyclic compounds,ketones and alcohols. The main compounds were caffeine,diethyl phthalate,dibenzofuran,dihydroactinidiolide,biphenyl,6,10-dimethyl-5,9-undecadien-2-one,phytone,cedrol,phenanthrene,1,2,3-Trimethoxybenzene. The two methods had the advantages and disadvantages,complement each other,and combined analysis could obtain the more comprehensive,more complete and more accurate information of the volatile components in Fu brick Tea.
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