大孔吸附树脂对鼠尾藻多酚的吸附解析性能研究

基金项目:

国家自然科学基金海外及港澳学者合作研究基金(51728902)

浙江省科技厅公益项目(LGF18D060002)。

详细信息

Study on Preparative Separation of Total Polyphenols from Sargassum thunbergii by Column Chromatography

1. Ocean College, Zhejiang University of Technology, Hangzhou 310014, China;
2. Marine Fishery Institute of Zhejiang Province, Wenzhou 325005, China;
3. College of Engineering and Applied Sciences, Memorial University, A1C5S7 Canada

摘要: 研究了XDA-1B大孔吸附树脂柱层析法对鼠尾藻多酚吸附解析条件。结果表明:最佳静态吸附条件为:温度30℃、样品中多酚浓度2.5 mg/mL,XDA-1B大孔吸附树脂适用于鼠尾藻多酚的分离纯化。上样流速2.0 mL/min,洗脱流速2.0 mL/min条件下,XDA-1B大孔吸附树脂对鼠尾藻多酚的吸附和脱附速率较快,2 h之后趋向吸附饱和脱附完全。Freundlich吸附经验公式适于其吸附曲线模拟。
- 鼠尾藻多酚 /
- XDA-1B大孔吸附树脂 /
- 吸附 /
- 解吸
Abstract: In this paper,the analytical conditions for the adsorption of polyphenols on the seaweed by XDA-1B macroporous resin were studied. The results showed that the optimal conditions for static adsorption were as follows:temperature 30℃,polyphenol concentration in the sample 2.5 mg/mL,and XDA-1B macroporous adsorption resin was suitable for the separation and purification of polyphenols from Sargassum thunbergii. With the sample flow rate of 2.0 mL/min and the elution flow rate of 2.0 mL/min,the adsorption and desorption rate of the polyphenols on the XDA-1B macroporous resin was faster and the adsorption equilibrium was reached after 2 h,and Freundlich adsorption empirical formula is suitable for the adsorption curve simulation.
- Sargassum thunbergii polyphenol /
- XDA-1B macroporous resin /
- adsorption /
- desorption