摘要:
为研究甘薯渣氢表雄酮(DHEA)的抑菌活性,本研究以马克斯克鲁维酵母、酿酒酵母、鼠李糖乳杆菌、大肠杆菌、枯草芽孢杆菌、炭疽杆菌、沙门氏菌、金黄色葡萄球菌为供示菌,采用生长曲线、最小抑菌浓度(MIC)、最低杀菌浓度(MBC)、生长曲线、扫描电镜研究DHEA的抑菌作用。结果表明,DHEA对供试菌的抑菌效果依次为:马克斯克鲁维酵母>大肠杆菌>金黄色葡萄球菌>炭疽杆菌>沙门氏菌>枯草芽孢杆菌>酿酒酵母>鼠李糖乳杆菌。最低抑制浓度(MIC)结果为:马克斯克鲁维酵母5.67 mg/mL,大肠杆菌9.82 mg/mL,金黄色葡萄球菌13.52 mg/mL,炭疽杆菌16.46 mg/mL,沙门氏菌17.68 mg/mL,枯草芽孢杆菌17.87 mg/mL,酿酒酵母20.82 mg/mL,鼠李糖乳杆菌无明显抑制作用。DHEA使供试菌生长周期缩短,并降低了菌体浓度;扫描电镜显示DHEA处理后菌体形态改变,细胞壁破裂,细胞内容物有溶出。研究表明,甘薯渣中DHEA对大部分细菌有抑制作用。
Abstract:
To study the antibacterial activity of Dehydroepiandrosterone(DHEA)in sweet potato residue,the antibacterial activity of DHEA was tested with Kluyveromyces marxianus,Saccharomyces cerevisiae,L. rhamnosus,E.coli,Bacillus subtilis,Bacillus anthraci,Salmonella,Staphylococcus aureus. Bacillus subtilis,Salmonella,Staphylococcus aureus and Bifidobacterium as the indicator bacteria. The antimicrobial activity of dehydroepiandrosterone was studied by turbidimetry,minimal inhibitory concentration(MIC),minimum bactericidal concentration(MBC),growth curve and scanning electron microscopy. The results showed that the bacteriostatic effect of DHEA on the tested bacteria was as follows:Kluyveromyces marxianus>Escherichia coli>Staphylococcus aureus>Bacillus anthracis>Salmonella>Bacillus subtilis>Saccharomyces cerevisiae>Lactobacillus rhamnosus. The minimum bactericidal concentration were as follows:Kluyveromyces marxianus 5.67 mg/mL,Escherichia coli 9.82 mg/mL,Staphylococcus aureus 13.52 mg/mL,Bacillus anthracis 16.46 mg/mL,Salmonella 17.68 mg/mL,Bacillus subtilis 17.87 mg/mL,Saccharomyces cerevisiae 20.82 mg/mL,Lactobacillus rhamnosus had no significant inhibitory effect. DHEA shortened the growth cycle of the test bacteria and reduced the cell concentration. Scanning electron microscopy showed that the morphology of the cells was changed after DHEA treatment,the cell wall ruptured and the cell contents were partially eluted. The research showed that DHEA in sweet potato residue had inhibitory effects on most bacteria.
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