Determination of the Total Phenolic Content from Cherry Wine by Folin-ciocalteu Method Optimized by Response Surface Methodology
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摘要: 本研究采用福林酚法测定樱桃酒中总酚的含量,并通过响应面法优化其工艺参数。在单因素实验基础上,以福林酚试剂用量、碳酸钠浓度、显色温度、显色时间和碳酸钠用量为影响因素,樱桃酒总酚含量为指标,响应面法进行分析和优化。结果表明,优化后得到最佳条件为:福林酚试剂2.1 mL、碳酸钠浓度63 g/L、碳酸钠用量8.32 mL,在25℃件下反应1.4 h,总酚含量为1085.2±0.16 mg/L。此回归模型拟合性良好,可用于预测总酚含量。根据此线性回归方程测定12个品种樱桃酒中总酚含量最高的为俄罗斯酸樱桃(1495.0 mg/L),雷尼含量最低(442.8 mg/L)。福林酚法操作简单,可用于不同品种樱桃酒总酚含量的测定。Abstract: The aim of this study was to determination of the total phenolic content from cherry wine by folin-ciocalteu method optimized by response surface methodology. Based on mono-factor experiments and response surface methodology, including folin-cioaclteu reagent, sodium carbonate concentration, reaction temperature, reaction time and sodium carbonate amount, the extraction conditons for improving tatal phenolic content from cherry wine were analyzed and optimized.Results showed that the optimum conditions for the total phenolic content were determined as follows:2.1 mL of folin-cioaclteu reagent, 8.32 mL of 63 g/L Na2 CO3 solution, reacting 1.4 h at 25℃.Under these conditions, the total phenolic content from cherry wine could be up to 1085.2 ±0.16 mg/L.The equation of linear regression corresponds with the experimental data and was enable to infer total phenolic content. And the highest total phenolic content in 12 varieties was Russian cherry (1495.0 mg/L) and the lowest content was Rainey (442.8 mg/L).This simple method provided an appropriate method for determining total phenolic content in different kinds of cherry wine.
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