利用大孔树脂对莼菜多糖脱色的工艺优化

张春燕; 吴美儒; 周毅峰; 唐巧玉

doi:10.13386/j.issn1002-0306.2018.15.028

利用大孔树脂对莼菜多糖脱色的工艺优化

1. 生物资源保护与利用湖北省重点实验室, 湖北民族学院, 湖北恩施 445000;
2. 生物科学与技术学院, 湖北民族学院, 湖北恩施 445000

基金项目:

湖北省教育厅重点项目(D20131902)。

详细信息

作者简介:
张春燕(1985-),女,硕士研究生,研究方向:野生动植物保护与利用,E-mail:364615070@qq.com。

通讯作者:
唐巧玉(1979-),女,博士研究生,副教授,研究方向:天然产物开发利用,E-mail:zhoujunye2009@163.com。

中图分类号: TS201.2
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出版历程
- 收稿日期: 2017-11-05
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-07-31

Optimization of Decolorization Condition of Brasinia schreiberi Polysaccharide Using Macroporous Resin

1. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Institute for Nationlities, Enshi 445000, China;
2. College of Bioscience and Technology, Hubei Institute for Nationlities, Enshi 445000, China

摘要

摘要: 为了研究莼菜多糖的脱色条件,本文以脱色率为主要测定指标,首先对比了不同类型的树脂对莼菜多糖的脱色效果,然后通过树脂种类、脱色温度、脱色pH的单因素实验和正交实验对脱色工艺参数进行了优化。结果表明:采用大孔树脂NKA-9树脂,在脱色温度55 ℃和脱色pH为4的条件下,莼菜多糖溶液的脱色率可达到79.22%,多糖保留率为67.54%,蛋白质去除率为55.75%。该脱色方法工艺简单,效果好,可用于莼菜多糖的脱色。
- 莼菜 /
- 多糖 /
- 大孔树脂 /
- 脱色条件
Abstract: In order to study the Brasinia schreiber polysaccharides decolonization condition,the decolorization capability of different kinds of resins was compared taking decolorizing rate as major selection standard. Then through the single factor experiment and orthogonal experiment of resins species,decolorization temperature and decolorization pH,the decolorization process parameters was optimized. The Result showed that NKA-9 resin had the best decolorization ability,under the decolorization temperature 55 ℃ and pH was 4,the decolonization rate of the Brasinia schreiber polysaccharides solution reached 79.22%,the Brasinia Schreiber polysaccharide retention rate was 67.54%,the protein removing rate was 55.75%. The decolorization process was simple,the effect was good,could be used as a decolorization method of Brasenia schreberi polysaccharides.
- Brasinia schreiber /
- polysaccharides /
- macroporous resin /
- decolonization condition