摘要:
采用超声辅助溶剂法提取桑葚花色苷,以吸附率和解吸率为指标,从大孔树脂HPD600、D101、LX1180、AB-8、DA201-C中筛选出性能较优的树脂对其进行纯化,最后对纯化后花色苷的稳定性进行研究。结果表明:以40%乙醇溶液(含0.1% HCl)为提取剂,料液比1:10,超声时间90 min,花色苷得率为2.9 mg/g。HPD600是纯化花色苷较为理想的树脂,除糖率67.6%,纯化后样品总花色苷含量为16.7%,色价为46.1,产品为紫黑色粉末,经高效液相色谱检测其含有约41.2%的矢车菊素-3-O-葡萄糖苷。随着温度、pH、光照强度的上升,花色苷的稳定性降低。Na+、Mg2+、Al3+对花色苷的稳定性影响不大,Cu+、Cu2+、Fe2+、Fe3+对花色苷有一定的破坏作用。因此,应在25℃、低pH、避光条件下对桑葚花色苷进行加工与保存,同时避免接触铜离子和铁离子。
关键词:
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桑葚
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花色苷
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提取
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大孔树脂
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稳定性
Abstract:
Anthocyanin from mulberry was extracted by ultrasonic assisted solvent method. According to adsorption rate and desorption rate,this paper selected good performance macroporous resin from HPD600,D101,LX1180,AB-8,DA201-C for purification,and the purified material's stability were studied. The results showed that the extraction rate of anthocyanin was 2.9 mg/g when 40% of ethanol(including 0.1% HCl)was used as extracting agent with a sample/solvent ratio 1:10,and the ultrasonic time 90 min. Among macroporous resins selected,the HPD600 was the best for the purification of anthocyanin. The sugar-removing ratio was 67.6%,the purity was 16.7%,the color value was 46.1,and the product was purple black powder. It contained about 41.2% of cornflower-3-O-glucoside. With the increasing of temperature,pH and illumination intensity,the stability of anthocyanin decreased. Anthocyanin was sensitive to Cu+,Cu2+,Fe2+,Fe3+,while Na+,Mg2+,Al3+ had a little influence on the stability of anthocyanins. Purified anthocyanin should be processed and stored in conditions of 25℃,low pH value,the dark and avoid contact cupric ion or iron ion.
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