竹荪多糖提取工艺及其抗氧化性

敢小双; 熊晨阳; 刘光荣; 薛燕; 董银卯

doi:10.13386/j.issn1002-0306.2018.13.039

竹荪多糖提取工艺及其抗氧化性

1. 无限极(中国)有限公司, 广东广州 510623;
2. 北京工商大学, 北京 100048

详细信息

作者简介:
敢小双(1981-),女,硕士研究生,研究方向:化妆品植物原料,E-mail:sunny.gan@infinitus-int.com。

通讯作者:
董银卯(1963-),男,博士,教授,研究方向:植物源化妆品功效成分研究与应用,E-mail:ymdong2008@163.com。

中图分类号: TS255.1
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出版历程
- 收稿日期: 2017-10-17
- 网络出版日期: 2020-11-26
- 刊出日期: 2018-06-30

Extraction Technology of Dictyophora Polysaccharide and its Antioxidative Activity

1. Wuxianji(China) Co., Ltd., Guangzhou 510623, China;
2. Beijing Technology and Business University, Beijing 100048, China

摘要

摘要: 以竹荪为原料,采用水提法提取竹荪多糖,比较了提取温度、pH、料液比和提取时间对竹荪多糖得率的影响,并且在单因素实验的基础上,采用正交实验,确定了水提法提取竹荪多糖的最佳工艺参数,即提取温度为90℃、pH为8.0、料液比为1:70 g/mL,时间为2.5 h。在上述提取条件下,提取竹荪多糖得率可达到13.09%。抗氧化实验结果表明,0.6 mg/mL的竹荪多糖对ABTS自由基的清除率可达92.76%,25 mg/mL的竹荪多糖对DPPH自由基的清除率可达70.55%。竹荪多糖提取工艺合理、可行,所提多糖具有较强的抗氧化性。
- 提取 /
- 竹荪多糖 /
- 得率 /
- 抗氧化性
Abstract: Dictyophora polysaccharide from Dictyophora was extracted by hot water extraction. The effects of extraction temperature,pH,the powder-to-water ratio and extraction time were studied by single factor experiment and orthogonal experiment. The results showed that optimal extract was at temperature 90℃,pH8.0,powder-to-water ratio 1:70 g/mL and extraction in 2.5 h. Under this condition,the yield of Dictyophora polysaccharide was 13.09%. The results of anti-oxidation experiments of Dictyophora polysaccharide showed that the scavenging rate of ABTS free radicals by the 0.6 mg/mL Dictyophora polysaccharide was 92.76% and the scavenging rate of DPPH free radical by the 25 mg/mL Dictyophora polysaccharide was 70.55% respectively. The extraction technology is reasonable and feasible,and the polysaccharide has stronger antioxidant activity.
- extraction /
- Dictyophora polysaccharide /
- yield /
- antioxidant activity