牡蛎蛋白酶解多肽降糖及抗氧化活性评价

赵荣涛; 王宁丽; 魏鉴腾; 裴栋; 刘晔玮; 邸多隆

doi:10.13386/j.issn1002-0306.2018.03.006

牡蛎蛋白酶解多肽降糖及抗氧化活性评价

赵荣涛^1,2,3,
王宁丽^2,3,
魏鉴腾^2,3,
裴栋^2,3, ,,
刘晔玮¹,
邸多隆^2,3

1. 兰州大学公共卫生学院营养与食品卫生学研究所, 甘肃兰州 730000;
2. 中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室, 甘肃兰州 730000;
3. 青岛市资源化学与新材料研究中心, 山东青岛 266000

基金项目:

中科院STS项目(KFJ-SW-STS-146)

青岛市民生科技计划项目(15-9-2-97-nsh)

宁波市科技富民项目(2016C10030)资助。

国家自然科学基金(21605149)

详细信息

作者简介:
赵荣涛(1992-),男,硕士,研究方向:天然产物中功能成分的筛选与评价,E-mail:m18363619665_1@163.com。

通讯作者:
裴栋(1982-),男,博士,副研究员,研究方向:天然产物化学,E-mail:dongpei@licp.cas.cn。

中图分类号: TS201.4
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出版历程
- 收稿日期: 2017-07-23
- 网络出版日期: 2020-12-25
- 刊出日期: 2018-01-31

Evaluation of hypoglycemic and antioxidant activity for peptides from oyster protein

1. Institute of Nutrition and Food Hygiene, Faculty of Public Health, Lanzhou University, Lanzhou 730000, China;
2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences(CAS), Lanzhou 730000, China;
3. Qingdao Center of Resource Chemistry&New Materials, Qingdao 266000, China

摘要

摘要: 目的:利用化学方法评价9种酶对牡蛎蛋白酶解所得多肽降血糖和抗氧化活性的影响,筛选最佳酶的种类。方法:以牡蛎蛋白酶解多肽对α-淀粉酶抑制率为指标评价其体外辅助降血糖活性,以DPPH·清除率、超氧阴离子抑制率为指标评价牡蛎蛋白酶解多肽的体外抗氧化活性,筛选出最佳用酶。结果:风味蛋白酶得到的多肽对α-淀粉酶的抑制效果最好,5 mg/mL时抑制率达到67.13%,IC₅₀值为3.806 mg/mL;糜蛋白酶酶解得到的多肽对DPPH·的清除率最高,2 mg/mL时清除率为58.09%,IC₅₀值为1.16 mg/mL;同时,糜蛋白酶酶解得到的多肽对超氧阴离子的抑制率也最高,2 mg/mL时抑制率为53.64%,IC₅₀值为1.75 mg/mL。结论:风味蛋白酶和糜蛋白酶酶解所得牡蛎多肽在降糖和抗氧化方面具有较大的潜力,为进一步开展牡蛎蛋白酶解多肽的研究提供参考。
- 蛋白酶 /
- 牡蛎 /
- α-淀粉酶抑制活性 /
- 降血糖活性 /
- 抗氧化活性
Abstract: Objective:To evaluate the effects of 9 enzymes on the hypoglycemic activity and antioxidant activity of peptides obtained from oyster protein by chemical method. Methods:As the evaluation indexes of hypoglycemic activity and antioxidant activity in vitro of peptides obtained from oyster protein,the inhibition rate of alpha amylase,the screening rate of DPPH·and the inhibition rate of superoxide anion were evaluated to screen the optimum protease. Results:The 5 mg/mL concentrations of peptides from oyster obtained by flavourzyme digestion had the most obvious inhibitory effect on α-amylase,and the IC₅₀ value was 3.806 mg/mL. The 2 mg/mL concentrations of peptides from oyster obtained by chymotrypsin digestion had the highest clearance rate of DPPH·,and IC₅₀ value was 1.16 mg/mL. Meanwhile,the 2 mg/mL concentrations of peptides from oyster obtained by chymotrypsin digestion had the highest inhibitory effect on superoxide anion,and the IC₅₀ value was 1.75 mg/mL. Conclusion:the peptides from oyster obtained by flavourzyme digestion and chymotrypsin digestion have great potentiality for hypoglycemic and antioxidant properties,and which are expected to provide a reference for the further study of peptides from oyster.
- protease /
- oyster /
- alpha amylase inhibitory activity /
- hypoglycemic activity /
- antioxidant activity