Optimization of microwave-assisted extraction technology of polysaccharide from tree peony seed meal and evaluation of antioxidant activity in vitro
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摘要: 研究牡丹籽粕多糖(PA)提取工艺及其抗氧化活性。采用微波辅助法提取超临界萃取牡丹籽油后的籽粕中的多糖,探讨微波处理时间、功率、粒度和料液比对多糖提取率的影响,通过正交实验优化提取工艺,用3,5-二硝基水杨酸盐法定量分析多糖含量,用DPPH和脂质过氧化法分析牡丹籽粕多糖的抗氧化活性。结果表明,各因素对多糖提取得率的影响大小依次为固液比>微波处理功率>籽粕粒度>微波处理时间,牡丹籽粕多糖的最佳提取工艺为:微波功率480 W,提取时间8 min,粒度120目,固液比1:25 (w/v),此条件多糖得率为9.21%。牡丹籽粕多糖溶液能有效的清除DPPH自由基,抑制卵黄组织匀浆的脂质过氧化作用。体外实验牡丹籽粕多糖具有一定程度的抗氧化能力,可成为一种新的天然抗氧化剂。本研究为牡丹籽粕的综合利用提供了理论依据与参考。Abstract: To develop polysaccharide by microwave-assisted extraction technique and test its antioxidant activity. The polysaccharide was extracted from tree peony seed meal, and that was determined by 3, 5-Dinitrosalicylic acid, to investigate extraction time, extraction power, seed meal granularity and the ratio of material to water. Based on these experiments, finally the orthogonal experiments were to optimize extraction condition. Meanwhile, the ability of polysaccharide to scavenge hydroxyl radical was studied to make a preliminary determination of its biological activity. The results showed the effect order of each factor on yield was as follows:stock ratio>extraction power>seed meal granularity>extraction time and the optimal extraction conditions of polysaccharide were 480 W microwave power for 8 min in combination with stock ratio 1:25 (m/v)and the size of granularity among 120 items, by which the yield of polysaccharide was up to 9.21%. The crude polysaccharide from tree peony seed meal could scavenge DPPH radical and inhibit lipid peroxidation of polyunsaturated fatty from yolk lipoprotein. Thus, the polysaccharide from tree peony seed meal could be developed as a new type of anti-oxidants. This study has provided a technical basis for further development of tree peony seed meal polysaccharide functions and utilization of tree peony resources.
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Keywords:
- tree peony /
- seed meal /
- polysaccharide /
- microwave /
- antioxidative activity
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[1] 史国安,焦封喜,焦元鹏,等.中国油用牡丹的发展前景及对策[J].中国粮油学报,2014,29(9):124-128. [2] 王瑞元.发展木本油料产业是提高我国食用油自给率的重要举措[J].粮食与食品工业,2016,23(4):1-4. [3] 史国安,郭香凤,金宝磊,等.牡丹籽油超临界CO2萃取工艺优化及抗氧化活性的研究[J].中国粮油学报,2013,28(4):93-98. [4] 常改,杨溢,霍飞,等.植物多糖的研究进展及保健功能[J].中国公共卫生,2003,19(11):1394-1395. [5] 吴雪辉,黄永芳,向汝莎,等.微波提取油茶饼粕中多糖的工艺研究[J].食品工业科技,2008,29(9):197-199. [6] 陈红,王大为,李侠,等.不同方法提取大豆多糖的工艺优化研究[J].食品科学,2010,31(4):6-10. [7] Matthäus B.Antioxidant activity of extracts obtained from residues of different oilseed[J].Journal of Agricultural and Food chemistry,2002,50(12):3444-3452.
[8] 谢明勇,聂少平.天然产物活性多糖结构与功能研究进展[J].中国食品学报,2013,10(2):1-11. [9] 方积年,丁侃.天然药物——多糖的主要生物活性及分离纯化方法[J].中国天然药物,2007,5(5):338-347. [10] Ganzler K,Szinai I,Salgo A.Effective sample preparation method for extracting biologically active compounds from different matrices by a microwave technique[J].Journal of Chromatography A,1990,520:257-262.
[11] 王磊,刘秀凤,邱芳萍,等.微波辅助提取玉米须多糖及其组成的研究[J].食品与生物技术学报,2009,28(1):72-75. [12] 柯乐芹,张东旭,肖建中.杏鲍菇深加工残渣多糖酶法微波辅助提取工艺优化[J].农业工程学报,2014,30(21):332-338. [13] 张利霞,常青山,侯小改,等.牡丹皮中多糖提取工艺的响应曲面法优化研究[J].山东农业科学,2015,47(6):118-124. [14] 王洪政,李媛媛,刘伟,等.响应面法优化牡丹果荚多糖提取工艺及其抗氧化活性评估[J].植物研究,2015,35(1):127-132. [15] 路祺,高悦,项凤影,等.牡丹种荚多糖提取工艺研究[J].植物研究,2015,35(1):154-157. [16] 谌志伟,滕文佳,刘选选,等.牡丹种仁多糖提取最佳工艺研究[J].中国酿造,2012,32(2):57-59. [17] 王顺利,任秀霞,薛璟祺,等.牡丹籽油成分、功效及加工工艺的研究进展[J].中国粮油学报,2016,31(3):139-146. [18] 毛善巧,李西俊.牡丹籽油的研究进展及油用牡丹综合利用价值分析[J].中国油脂,2017,42(5):123-126. [19] 彭长连,陈少薇,林植芳,等.用清除有机自由基DPPH法评价植物抗氧化能力[J].生物化学与生物物理进展,2000,27(7):658-661. [20] Larrauri J A,Sanchez-Moreno C,Saura-Calixto F. Effect of temperature on the free radical scavenging capacity of extracts from red and white grape pomace peels[J].Joural of Agricltural Food Chemstry,1998,46(7):2694-2697.
[21] 张尔贤,俞丽君,周意琳,等.Fe2+诱发脂蛋白PUFA过氧化体系及对若干天然产物抗氧化作用的评价[J].生物化学与生物物理学报,1996,28(2):218-222. [22] 史国安,郭香凤,高双成,等.牡丹花发育过程中花瓣抗氧化活性的变化[J].园艺学报,2009,36(11):1685-1690.
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