Abstract: Rice glutelin was prone to thermal aggregation, and ionic strength had obvious effect on its thermal aggregation.Here, the effect laws and mechanisms of rice glutelin thermal aggregation by Na Cl were revealed.When Na Cl concentration increased from 0⁰.15 mol·L^-1, solubility of rice glutelin treated by heat decreased from 13.78% to 12.49%, which seemed not to be obvious, but the solubility was 10.62% when Na Cl concentration was 0.30 mol·L^-1, and it appeared to be turbid.The turbidity of rice glutelin by heat treatment increased significantly from 0.052 to 0.191 when Na Cl concentration increased from0⁰.30 mol·L^-1, average particle size increased from 168 nm to 273 nm, and an aggregation peak rised, Zeta potential changed from -14.4 to-5.61, and hydrophobicity increased from 329.16 to 535.80, there was a slight blue shift for fluorescence emission spectrum of rice glutelin by heat treatment, and fluorescence intensity increased. Na Cl affected surface charge and hydrophobicity of rice glutelin by heat treatment, and promoted its thermal aggregation when concentration of Na Cl was more than 0.15 mol·L^-1.