Antioxidant activity of the selenium-riched rice peptides in vivo
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摘要: 目的:比较普通大米肽、富硒大米肽和硒代蛋氨酸的体内抗氧化活性。方法:将60只老龄（12月龄）雄性昆明小鼠和10只少龄（1月龄）雄性昆明小鼠分成7组（每组10只）:老龄模型组（M组）、少龄对照组（YC组）、普通大米肽组（400 mg/kg·bw,Ⅰ组）、富硒大米肽组（400 mg/kg·bw,Ⅱ组）、硒代蛋氨酸组（2.5μg/kg·bw,Ⅲ组）、富硒大米肽+硒代蛋氨酸组（200 mg/kg·bw+1.225μg/kg·bw,Ⅳ组）和白藜芦醇组（10 mg/kg.bw,PC组）,其中富硒大米肽组、硒代蛋氨酸组以及富硒大米肽+硒代蛋氨酸组中的硒含量一致,连续灌胃30 d。结果显示:与M组相比,YC组、Ⅱ组、Ⅲ组、Ⅳ组、PC组小鼠血清中超氧化物歧化酶（SOD）活性极显著提高（p<0.01）;YC组、Ⅰ组、Ⅳ组的还原型谷胱甘肽（GSH）含量显著增加（p<0.05）,Ⅱ组和PC组GSH含量极显著增加（p<0.01）;YC组、Ⅱ组、PC组的过氧化氢酶（CAT）活性显著增加（p<0.05）;Ⅲ组、Ⅳ组的肝匀浆中谷胱甘肽过氧化物酶（GSH-Px）活性显著提高（p<0.05）,Ⅱ组的GSH-Px活性极显著增加（p<0.01）;Ⅱ组、Ⅳ组、PC组的T-AOC显著增加（p<0.05）;YC组、Ⅱ组的丙二醛（MDA）显著减少（p<0.05）,PC组的MDA极显著减少（p<0.01）;YC组、PC组的GSH显著增加（p<0.05）,Ⅱ组的GSH极显著增加（p<0.01）,以及胸腺、脾脏指数等指标均有不同程度地改善。其中所有指标以富硒大米肽组效果最好,抗氧化活性显著高于同剂量的普通大米肽,大多数指标也显著优于同等硒含量的硒代蛋氨酸组。结论:富硒大米肽中的硒与肽对增强抗氧化能力有一定的增效作用。Abstract: Objective: To compare the antioxidant activity of the ordinary rice peptides, selenomethionine and selenium-riched peptides.Methods: The mice ( 12 months of age) were administrated by the gavage of ordinary rice peptides ( 400 mg/kg·bw, Ⅰgroup) , selenium-riched peptides ( 400 mg/kg·bw, Ⅱ group) , selenomethionine ( 2.5 μg/kg·bw, Ⅲ group) , selenium-riched peptides ( 200 mg/kg·bw) + selenomethionine ( 1.225 μg/kg·bw, Ⅳ group) and resveratrol ( 10 mg/kg·bw, PC group) for 30 d respectively in different groups. The selenium content of Ⅱ group, Ⅲ group and Ⅳ group was same. Results: It showed that compared with the model group, YC group、Ⅱ group、Ⅲ group、Ⅳ group、PC group showed highly significant improvement on SOD in the serum ( p < 0.01) ; YC group、Igroup、Ⅳ group showed significant improvement on GSH in the serum ( p < 0.05) , Ⅱgroup and PC group showed highly significant improvement on GSH ( p < 0.01) ; YC group、Ⅱ group、PC group showed significant improvement on CAT in the serum ( p < 0.05) ; Ⅲ group、Ⅳ group showed significant improvement on GSH-Px in liver homogenateand ( p < 0.05) , II group showed highly significant improvement on GSH-Px ( p < 0.01) ; Ⅱ group、Ⅳ group、PC group showed significant improvement on T-AOC in liver homogenateand ( p < 0.05) ; YC group、Ⅱ group showed significant decrease on MDA in liver homogenateand ( p < 0.05) , PC group showed highly significant decrease on MDA ( p < 0.01) ; YC group、PC group showed significant improvement on GSH in liver homogenateand ( p < 0.05) , II group showed highly significant improvement on GSH ( p < 0.01) , and thymus and spleen index showed different degrees of improvement.The effect of selenium-riched peptides was almost the best, which the antioxidant activities of selenium-riched peptides were significantly higher than that of the ordinary rice peptides and most indexes of selenium-riched peptides were also significantly better than the same selenium content of selenomethionine. Conclusion: It suggested that selenium and peptides in selenium-riched rice peptides had a certain synergy effect on improving antioxidant activities.
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Keywords:
- selenium-riched rice peptide /
- selenomethionine /
- antioxidant /
- synergy
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