Optimization of high density fermentation conditions of Kluyveromyces marxianus
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摘要: 对影响马克斯克鲁维酵母高密度发酵的培养基营养成分和培养条件展开分析研究。单因素实验发现,YPD作为基础培养基有利于马克斯克鲁维酵母的增殖;培养基成分响应面分析和培养条件正交实验结果表明,当培养基成分为蔗糖67.37 g/L,酵母浸粉29.7 g/L,玉米浆15.61 g/L,KH2PO44.13 g/L,MgSO40.3 g/L,初始pH为6.0、发酵温度为30℃、搅拌速度为160 r/min时,发酵培养18 h马克斯克鲁维酵母的生物量最大,为（9.34±0.12）g/L。进一步进行乳饮品发酵实验,优化培养的马克斯克鲁维酵母与乳源培养基培养的马克斯克鲁维酵母,在菌种的生物量和乳饮品的口感风味上无明显差异。因此,优化后的高密度发酵培养基配方和工艺条件适宜于马克斯克鲁维酵母菌的高密度发酵。
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关键词:
- 马克斯克鲁维酵母 /
- 高密度发酵 /
- 增殖速率 /
- 固相微萃取-气相色谱-质谱法
Abstract: The effects of high density fermentation Kluyveromyces marxianus on nutritional component and cultivation condition of culture medium was studied. Single factor experiment revealed that YPD as the basic medium for the proliferation of Kluyveromyces marxianus. When the medium was 67.37 g/L sucrose, yeast extract 29.7 g/L, corn steep 15.61 g/L, KH2PO4 4.13 g/L, MgSO40.3 g/L, initial pH6, fermentation temperature was 30 ℃, stirring speed was 160 r/min, the fermentation biomass of 18 h Kluyveromyces marxianus was ( 9.34 ± 0.12) g/L by response surface analysis and culture condition orthogonal test.Fermented dairy experiments showed that there was no obvious difference in bacterial biomass, taste and flavor of fermented dairy produced by high density and skimmed milk medium culture Kluyveromyces marxianus.Therefore, the optimized high density fermentation medium formula and process conditions were suitable for the cultivation of the yeast Kluyveromyces marxianus. -
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