Antioxidant activity of pitaya peel beverage fermented by lactic acid bacteria in vitro
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摘要: 为开发火龙果皮益生菌发酵新产品,实现对其增值利用,将火龙果皮通过植物乳杆菌发酵,采用不同的自由基体系对其发酵产品的体外抗氧化活性进行测定,并对其发酵前后色素、多糖、总黄酮含量变化进行分析。结果表明,发酵前、后火龙果皮汁对超氧阴离子（O2-·）、DPPH自由基具有较强的清除能力,对羟基自由基（·OH）、亚硝酸根离子（NO2-）也具有一定的清除作用;且清除能力均随浓度增大而增强;发酵后对羟基自由基（·OH）、超氧阴离子（O2-·）、亚硝酸根离子（NO2-）、DPPH自由基的IC50分别为0.77、0.33、0.47、0.17 m L/m L,较发酵前分别减少25.96%、28.26%、47.78%、57.50%;相同浓度下,发酵可有效提高火龙果皮汁的自由基清除能力,但提高幅度随浓度增大而逐渐减小;发酵后色素和总黄酮浓度较发酵前下降,但多糖含量增高。说明火龙果皮汁经植物乳杆菌发酵可进一步提高其抗氧化能力,为火龙果皮保健功能及益生菌发酵产品开发提供理论依据。Abstract: To develop probiotic fermented new products and realize the value-added utilization of pitaya peel, the antioxidant ability of pitaya peel beverage fermented by Lactobacillus plantarum was investigated by means of different free radicals antioxidant experiments in vitro, and the content of pigment, polysaccharide, total flavonoid of unfermented and fermented were analyzed.The results showed that the pitaya peel beverage both unfermented and fermented had a strong scavenging ability to superoxide anion ( O2-·) , DPPH free radicals, and a certain scavenging ability to hydroxyl free radical (·OH) , nitrite ion ( NO2-) , within a certain range, the clearance rate increased with the increase of sample concentration.The IC50 of fermented pitaya peel beverage to hydroxyl free radical (·OH) , superoxide anion ( O2-·) , nitrite ion ( NO2-) and DPPH free radicals respectively were 0.77, 0.33, 0.47, 0.17 m L/m L, which decreased by 25.96%, 28.26%, 47.78%, 57.50% respectively compared with unfermented.Fermentation could effectively improved the free radical scavenging capacity of pitaya peel beverage at the same concentration, but the increase amplitude decreased gradually with the increase of concentration, pigment and total flavonoid concentration decreased after fermentation, but the content of polysaccharide increased.This suggested that fermented by Lactobacillus plantarum could further improve the antioxidant capacity of pitaya peel, provided theoretical basis for the development of pitaya peel hygienical and probiotic fermented products.
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Keywords:
- pitaya peel /
- Lactobacillus plantarum /
- fermentation /
- antioxidant
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[1] 曾建飞.中国植物志 (第五十二卷第一分册) [M].北京:科学出版社, 1999:275-300. [2] 陈晓旭, 易建勇, 郭小宁, 等.火龙果加工技术研究进展[J].农产品加工, 2014 (5) :48-53. [3] Alireza O, Rokiah M Y, Shahin R, et al.Anti-diabetic activity of red pitaya (Hylocereus polyrhizus) fruit[J].Royal Society of Chemistry, 2014, 4:62978-62986.
[4] Femanda R M, Claudia B, Carolinne O D, et al.Evaluation of the chemical characteristics and rheological behavior of pitaya (Hylocereus undatus) peel[J].EDP Sciences, 2014, 69:381-390.
[5] 蔡永强, 向青云, 陈家龙, 等.火龙果的营养成分分析[J].经济林研究, 2008, 26 (4) :53-56. [6] 王壮, 王立娟, 蔡永强, 等.火龙果营养成分及功能性物质研究进展[J].中国南方果树, 2014, 43 (5) :25-29. [7] 张福平.火龙果的营养保健功效及开发利用[J].食品研究与开发, 2002, 23 (3) :49-50. [8] Lee-Hoom H, Nadratul Wahidah Binti A L.Nutritional composition, physical properties, and sensory evaluation of cookies prepared from wheat flour and pitaya (Hylocereus undatus) peel flour blends[J].Cogent Food&Agriculture, 2016, 2:1136369.
[9] Jamilah B, Shu C E, Kharidah M, et al.Physico-chemical characteristics of red pitaya (Hylocereus polyrhizus) peel[J].International Food Research Journal, 2011, 18:279-286.
[10] Song G Haizhao, Chu Qiang, Xu Dongdong, et al.Purified betacyanins from Hylocereus undatus peel ameliorate obesity and insulin resistance in high-fat-diet-fed mice[J].Agricultural and Food Chemistry, 2016, 64:236-244.
[11] 赵文红, 梁彬霞, 白卫东, 等.火龙果果皮色素的研究进展[J].中国调味品, 2012, 37 (8) :111-115. [12] 杨志娟, 曾真, 吴晓萍.火龙果皮原花青素提取纯化及定性分析[J].食品科学, 2015, 36 (2) :75-79. [13] 王晓波, 何晓燕, 王梅, 等.火龙果皮总黄酮提取与体外抗氧化作用研究[J].食品工业科技, 2011, 32 (11) :156-159. [14] 张玉锋, 孙丽平, 庄永亮, 等.火龙果皮中膳食纤维含量及其物理化学特性[J].食品科学, 2012, 33 (19) :164-167. [15] 王雪, 丁金龙, 黄苇, 等.火龙果皮中果胶的提取及其结构研究[J].中国食品添加剂, 2015 (3) :102-106. [16] 罗萍, 师莉莎, 刘华忠.火龙果汁体外抗氧化能力研究[J].广东农业科学, 2013 (20) :93-96. [17] 董银卯, 何聪芬, 王领, 等.火龙果酵素生物活性的初步研究[J].食品科技, 2009, 34 (3) :192-196. [18] Zhuang Yongliang, Zhang Yufeng, Sun Liping.Characteristics of fibre-rich powder and antioxidant activity of pitaya (Hylocereus undatus) peels[J].International Journal of Food Science and Technology, 2012, 47:1279-1285.
[19] 赵兰, 苏小建, 王鹏程, 等.火龙果皮化学成分的研究[J].中成药, 2017, 39 (1) :111-115. [20] 黎海利, 刘锴栋, 袁长春, 等.红肉火龙果果皮色素提取工艺优化及其抗氧化活性[J].食品与发酵工业, 2014, 40 (12) :203-208. [21] Femanda R M, Claudia B, Caroline O D, et al.Antioxidant properties, quantification and stability of betalains from pitaya (Hylocereus undatus) peel[J].Food Technology, 2015, 45 (2) :323-328.
[22] 王晓波, 钟婵君, 刘冬英, 等.火龙果皮总黄酮对油脂抗氧化作用的研究[J].食品研究与开发, 2012, 33 (3) :19-22. [23] Luo Hui, CAI Yongqiang, Peng Zhijun, et al.Chemical composition and in vitro evaluation of the cytotoxic and antioxidant activities of supercritical carbon dioxide extracts of pitaya (dragon fruit) peel[J].Chemistry Central Journal, 2014, 8:1.
[24] Osman N, Adawi D, Ahrńe S, et al.Endotoxin-and d-galactosamine induced liver injury improved by the administration of Lactobacillus, Bifidobacterium and blueberry[J].Digestive and Liver Disease, 2007, 39 (9) :849-856.
[25] Tomomi K, Takashi K, Choa A, et al.Radical scavenging capacities of saba-narezushi, Japanese fermented chub mackerel, and its lactic acid bacteria[J].Food Science and Technology, 2012, 47 (1) :25-30.
[26] Anoectochilus F H, Ng C C, Wang C Y, et al.Lactic acid bacterial fermentation on the production of functional antioxidant herbal[J].Journal of Bioscience and Bioengineering, 2011, 111 (3) :289-293.
[27] 宋晓辰, 彭新颜, 李凤梅, 等.植物乳杆菌NDC75017抗氧化活性研究[J].食品科学, 2014, 35 (21) :106-112. [28] 王曦, 罗霞, 许晓燕, 等.不同乳酸菌菌株抗氧化能力的比较研究[J].食品科学, 2010, 31 (9) :197-201. [29] 严榕, 傅昌年, 周朝辉.植物乳杆菌对亚硝酸根离子的清除作用[J].食品工业, 2003 (6) :40-41. [30] 刘双环, 李欢, 王聪, 等.荷叶黄酮对乳酸杆菌和双歧杆菌抗氧化能力的协同作用研究[J].天然产物研究与开发, 2015 (27) :1712-1716. [31] 胡坤, 邢锐伟, 黎景恒, 等.热处理对红肉火龙果色素稳定性及其清除羟基自由基能力的影响[J].现代食品科技, 2012, 28 (8) :954-948. [32] 李志洲, 陈均志.大枣多糖的抗氧化性研究[J].食品工业科技, 2007 (4) :115-117. [33] 刘长江, 潘松, 梁爽.软枣猕猴桃多糖的体外抗氧化活性[J].食品科学, 2012, 33 (23) :79-82. [34] 陈燕, 蒋志涛, 陈晓峰, 等.苯酚-硫酸法测定黄芪口服液和注射液中多糖含量[J].现代中药研究与实践, 2015, 29 (5) :64-65. [35] 易艳东, 余南才, 马威, 等.马齿苋水提液中的总黄酮的含量测定[J].中国医院药学杂志, 2009, 29 (5) :424-425. [36] 李广富, 陈伟, 范路平, 等.茶树菇多糖酸奶的抗氧化活性与抗衰老研究[J].现代食品科技, 2015, 31 (10) :140-145. [37] 白明, 孟祥晨.益生菌抗氧化活性及菌体抗氧化相关成分的分析[J].食品与发酵工业, 2009, 35 (5) :6-10. [38] Nagendra Prasad K, Yang B, Dong X H, et al.Flavonoid contents and antioxidant activities from Cinnamomum species[J].Innovative Food Science and Emerging Technologies, 2009, 10 (4) :627-632.
[39] 赵金伟, 李范洙, 张先.苹果梨酚类物质抗氧化活性研究[J].食品科学, 2010, 31 (17) :170-172. [40] 裘纪莹, 陈相艳, 刘孝永, 等.银杏花粉及其发酵饮料的总黄酮含量及总黄酮测定方法比较[J].江苏农业科学, 2015, 43 (10) :371-373. [41] 王英, 周剑忠, 黄开红, 等.益生菌干酪乳杆菌FM10-3产胞外多糖培养条件的优化[J].中国乳品工业, 2014, 42 (2) :9-11. [42] Liu Chinfeng, Tseng Kuochuan, Chiang Shenshih, et al.Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides[J].Journal of the Science of Food and Agriculture, 2011, 91 (12) :2284-2291.
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