Ultrasonic extraction of Acer truncatum Bunge oil and the analysis of the nervonic acid content during the germination
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摘要: 以元宝枫种仁为研究对象,油脂得率为评价指标,通过超声功率、超声时间、料液比单因素实验,建立元宝枫油超声提取方法。同时优化元宝枫种子萌发温度,利用气相色谱法分析了萌发前后种仁油中神经酸含量的变化,并通过MTT法分析了萌发前后元宝枫种仁油对神经细胞的影响。结果表明:通过单因素确定的最佳提取工艺条件为:超声功率200 W、超声温度25℃、料液比1∶12。元宝枫种子人工萌发的最佳温度是4℃,气相色谱分析显示萌发后元宝枫种仁油中神经酸含量显著上升,可达萌发前的1.5倍。结果显示元宝枫种仁油能够促进神经细胞的增殖,且萌发后的种仁油作用更强。Abstract: Firstly, the effect of ultrasonic power, extracting temperature and the solid-liquid ratio on oil yield of Acer truncatum Bunge oil was established. The appropriate temperature of artificial germination of seeds was also investigated. The content of nervonic acid in the seeds oil was detected by GC analysis. The effect of the seed oil on the proliferation of nerve cells was detected by MTT.As the results shown, the optimal extraction conditions were as follows: ultrasonic power 200 W, extracting temperature 25 ℃, the solid-liquid ratio 1∶ 12.The seeds of Acer truncatum Bunge were germinated at 4 ℃. The results of GC analysis showed that the content of nervonic acid was significantly increased to 1.5 fold after the germination. Nervonic acid could promote the growth of nerve cells, and the oil from the germinated seeds of Acer truncatum Bunge was stronger than the dormant seed oil.
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Keywords:
- Acer truncatum Bunge /
- ultrasonic-assisted extraction /
- nervonic acid /
- germination /
- proliferation
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