Study on the liquid fermentation conditions of glucoamylase by Monascus purpureus
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摘要: 通过培养基和发酵条件的优化提高紫色红曲霉G6液态发酵产糖化酶活力。研究接种菌龄、碳源、氮源、金属离子、培养基初始pH、接种量、装液量、摇床转速以及温度等对产酶的影响。结果表明,最佳培养基配方（w/v）为大米粉8%,蛋白胨1%,KCl0.1%,ZnSO40.01%,FeSO40.005%,MnSO40.015%,培养基初始pH为4.0;最适发酵条件:装液量50mL/250mL,转速150r/min,温度32℃,接种量12%（v/v）;在此条件下发酵10d,糖化酶活力为1652.36U/mL比优化前（109.54U/mL）提高了14.08倍。Abstract: The production conditions of glucoamylase through liquid-state fermentation by Monascus purpureus G6 were optimized.Based on results of single factor tests, the optimal conditions for glucoamylase production were 8% rice powder, 1%peptone, 0.1% KCl, 0.01% ZnSO4, 0.005% Fe SO4, 0.015% MnSO4, 50 m L/250 m L loading medium volume ( v/v) , 12%inoculation amount ( v/v) and rotation speed of shaking incubator was 150 r/min, initial p H value of culture medium was 4.0, fermentation temperature was 32 ℃.The fermentation was carried out under these conditions for 10 days to obtain the maximum glucoamylase activity of 1652.36 U/m L, which was 14.08 times higher than that without optimization of fermentation condition.
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Keywords:
- Monascus purpureus /
- glucoamylase /
- culture medium /
- fermentation conditions
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