Biological characteristics and exoenzymes analysis of a mutated strain Bacillus amyloliquefaciens NCU116-1
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摘要: 以解淀粉芽孢杆菌诱变菌株NCU116-1为出发菌,对其生物学特性和培养特征进行研究。并用国标法、羧甲基纤维素糖化法等对该突变株的主要酶系的发酵时间和酶学性质进行分析测定。结果表明,淀粉酶、糖化酶和纤维素酶的最佳发酵时间是40 h;中性蛋白酶最佳发酵时间是44 h,果胶酶最佳发酵时间是42 h。中性蛋白酶在50℃时活力最高,Mn2+对其有激动作用,最适宜酶解温度为4045℃、适宜酶解p H为8;糖化酶在35℃时活力最高,Cu2+、Fe3+和Mn2+对其有激动作用,最适宜酶解温度为35℃左右、适宜酶解p H为8.0;果胶酶在40℃时活力最高,Mn2+和Ca2+对其有激动作用,最适宜酶解温度为3540℃、适宜酶解p H为6.0;纤维素酶在50℃时活力最高,Ca2+和Cu2+对其有激动作用,最适宜酶解温度为4045℃,适宜酶解p H为7.0。该菌株生产的胞外酶符合水酶法提取植物油脂复合酶制剂的要求,本文为该类菌株的应用奠定理论基础。Abstract: The biological characteristics and culture characteristics of Bacillus amyloliquefaciens NCU116-1 were studied.Nation-standard method and carboxymethyl cellulose saccharification method were used to analyze the enzymes production fastigium and their properties.The result showed that the optimal fermentation time of amylase, amylase and cellulase was 40 h.The optimum fermentation time of the neutral protease was 44 h. The optimum fermentation time of pectinase was 42 h, The neutral protease had the maximum activity at 50 ℃.Mn2 +was an activator of neutral protease.Its optimum temperature and p H value were 40~45 ℃ and 8 respectively.The glucoamylase had the maximum activity at 35 ℃, and was activated by Ca2 +, Fe3 +and Mn2 +.Its optimum temperatures and p H value were 35~40 ℃ and 8.0 respectively.The pectase had the maximum activity at40 ℃, and was activated by Mn2 +and Ca2 +. Its optimum temperatures and p H value were 35 ~ 40 ℃ and 6.0 respectively. The cellulase had the maximum activity at 50 ℃, and was activated by Ca2 +and Cu2 +.Its optimum temperatures and p H value were40~45 ℃ and 7.0 respectively.The extracellular enzymes produced by the strain meet the requirements of aqueous enzymatic method and this paper lays a theoretical foundation for the application of this kind of strain.
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Keywords:
- Bacillus amyloliquefaciens /
- fermentation time /
- complex enzyme /
- enzyme analysis
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[1] Rosenthal A, Pyle D L, Niranjan K.Aqueous and enzymatic processes for edible oil extraction[J].Enzyme and Microbial Technolog, 1996, 19 (6) :402-420.
[2] 于慧君, 周建平, 黄耀武.酶对油菜籽油水酶法提取率的影响研究[J].现代食品科技, 2011 (3) :328-331. [3] 赵玮, 王大为, 李倩.水酶法提取玉米胚芽油工艺优化[J].食品科学, 2010 (24) :206-209. [4] 刘志强, 贺建华, 曾云龙, 等.酶及处理参数对水酶法提取菜籽油和蛋白质的影响[J].中国农业科学, 2004 (4) :592-596. [5] 曾诚, 马毛毛, 肖彦骏, 等.解淀粉芽孢杆菌Z16的产酶条件优化及应用[J].食品工业科技, 2016, 37 (3) :196-200. [6] 肖彦骏, 曾诚, 马毛毛, 等.用于水酶法提取中碳链油脂菌株的筛选与鉴定[J].食品工业科技, 2015, 36 (22) :173-178. [7] Deb P, Talukdar S A, Mohsina K, et al.Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001[J].Springerplus, 2013, 2 (154) .
[8] Schallmey M, Singh A, Ward O P.Developments in the use of Bacillus species for industrial production[J].Canadian Journal of Microbiology, 2004, 50 (1) :1-17.
[9] GB/T 23527-2009, 蛋白酶制剂[S].北京:中国标准出版社, 2009. [10] GB/T 23535-2009, 脂肪酶制剂[S].北京:中国标准出版社, 2009. [11] GB 8275-2009, 食品添加剂α-淀粉酶制剂[S].北京:中国标准出版社, 2009. [12] GB 8276-2006食品添加剂糖化酶制剂[S].北京:中国标准出版社, 2006. [13] GB 1502-92, 食品添加剂果胶酶制剂[S].北京:中国轻工业出版社, 1993. [14] 穆春雷, 武晓森, 李术娜, 等.低温产纤维素酶菌株的筛选、鉴定及纤维素酶学性质[J].微生物学通报, 2013 (7) :1193-1201. [15] 王素梅, 王璋.水酶法提油工艺对玉米胚油质量的影响[J].食品科技, 2002 (9) :9-11. [16] 陈营, 桂远明, 陈晓刚.几种离子和有机化合物对枯草芽胞杆菌蛋白酶活力的影响[J].大连海洋大学学报, 2001, 16 (2) :125-129. [17] 程明, 崔承彬, 李长伟, 等.化学诱变技术在微生物育种研究中的应用[J].国际药学研究杂志, 2009, 36 (6) :412-417. [18] 熊俐, 杨跃寰, 胡洋.物理诱变技术在食品工业微生物育种上的应用进展[J].江苏农业科学, 2010 (5) :457-459. [19] 马宏颖.中性蛋白酶高产菌株的诱变选育及发酵条件研究[D].保定:河北农业大学, 2008. [20] 朱非, 王珊, 周培瑾.低温酶冷适应的分子机制及其在生物技术中的应用[J].微生物学报, 2002 (5) :640-644. [21] 林祥木, 童金秀, 陈汉清, 等.产纤维素酶菌株的筛选及产酶条件的选择[J].福建农业大学学报, 2003 (4) :510-513. [22] 陈曦, 赵建国.饲用酶高产菌株的筛选[J].饲料工业, 2006 (6) :14-15. [23] 宋鹏, 黄亮, 陈亮.产复合酶枯草芽孢杆菌QLB6的激光诱变育种[J].食品科学, 2011 (9) :222-224. [24] 王水顺, 林进哲, 张金玮, 等.复合酶高产菌株选育的研究[J].药物生物技术, 2001 (6) :317-321. [25] 胡学智, 王俊.蛋白酶生产和应用的进展[J].工业微生物, 2008 (4) :49-61.
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