Effects of solid concentrations on rheological property of wheat gluten protein hydrolyzed by acid protease
-
摘要: 本文较系统地研究了PR23酸性蛋白酶催化不同浓度小麦面筋蛋白水解过程中酶解产物的表观现象、表观粘度、剪切应力和流变特性。研究结果表明:酶解体系中小麦面筋的浓度越高,反应液的p H上升越多,水分活度下降幅度越大。在整个酶解反应过程中,低底物浓度(8%32%)酶解产物的表观粘度和剪切应力均会随着酶解时间的延长呈先升高后降低趋势;高底物浓度(40%)酶解产物的表观粘度和剪切应力均随着酶解时间的延长呈降低趋势。Ostwald-de waele模型可以很好的拟合酸性蛋白酶催化不同固形物浓度小麦面筋蛋白酶解产物。Abstract: Apparent viscosity, shear stress and rheological properties of wheat gluten hydrolysates catalyzed by PR23, an acid protease, at different solid concentration were studied. Results showed that the increased solid concentrations of Glutenhydrolysates resulted in higher p H and lower water acitivity. Apparent viscosity and shear stress of wheat hydrolysates increased after 12 h of hydrolysis, and then decreased for solid concentration of 8%, 16%, 24% and 32%. The apparent viscosity and shear stress decreased with the reaction time for solid concentration of 40% during the whole hydrolysis process.Ostwald-de waele model can fit the wheat protein hydrolysis process for all solid concentration.
-
Keywords:
- wheat gluten /
- solid concentration /
- rheological property
-
[1] 王亚平, 安艳霞.小麦面筋蛋白组成、结构和功能特性[J].粮食与油脂, 2011 (1) :1-4. [2] 张海华, 周惠明.小麦面筋蛋白结构及其改性研究[J].粮食与油脂, 2010 (9) :1-3. [3] 彭海萍, 王兰.小麦面筋蛋白在食品工业中的应用[J].西部粮油科技, 2002, 27 (2) :32-33. [4] Cui C, Hu Q, Ren J, et al.The effect of lactic acid bacteria fermentation on the antioxidant activity of wheat gluten pancreatin hydrolysates[J].International Journal of Food Science&Technology, 2014, 49 (4) :1048-1054.
[5] Hardt N A, Goot A J V D, Boom R M.Influence of high solid concentrations on enzymatic wheat gluten hydrolysis and resulting functional properties[J].Journal of Cereal Science, 2013, 57 (3) :531-536.
[6] Hardt N A, Janssen A E M, Boom R M, et al.Factors impeding enzymatic wheat gluten hydrolysis at high solid concentrations[J].Biotechnology&Bioengineering, 2014, 111 (7) :1304-1312.
[7] 大连轻工业学院等八大院校.食品分析[M].北京:中国轻工业出版社, 1994:177-180. [8] 龙肇.蛋白质/多糖交互作用对高乳脂乳浊液稳定性的影响及作用机理研究[D].广州:华南理工大学, 2014. [9] Hansson T, Andersson M, Wehtje E, et al.Influence of water activity on the competition betweenβ-glycosidase-catalysed transglycosylation and hydrolysis in aqueous hexanol[J].Enzyme&Microbial Technology, 2001, 29 (9) :527-534.
[10] 王章存, 陆杰, 李乐静, 等.谷朊粉的有限酶解对其分散性及胶黏性的影响[J].食品工业科技, 2012 (5) :119-122. [11] Bae H O, Kang K, Lee J, et al.Regularity for Ostwald-de Waele type shear thickening fluids[J].Nonlinear Differential Equations&Applications Nodea, 2015, 22 (1) :1-19.
[12] Vajravelu K, Prasad K V, Datti P S, et al.Convective flow, heat and mass transfer of Ostwald-de Waele fluid over a vertical stretching sheet[J].Journal of King Saud University, 2017, 29 (1) :57-67.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量: