Progress of aqueous two-phase extraction technique
-
摘要: 双水相萃取技术是一种易于放大、操作条件温和、可连续化操作的新型分离技术。本文综述了新近发展的不同的双水相体系（聚合物-盐-水、有机溶剂-盐-水、离子液体-盐-水、离子液体-表面活性剂-盐-水）的性质,及萃取（提取）蛋白质、氨基酸、抗生素和中药材中小分子有效成分等的影响因素;总结了双水相萃取与其它技术的联用,展望了双水相体系的应用前景。Abstract: Extraction with aqueous two-phase system ( ATPS) is a novel separation technique, which shows some advantages including easy scale-up, mild conditions and continuous operation.The properties and applications of newly developed different ATPSs ( polymer-salt-water, organic solvent-salt-water, ionic liquid-salt-water, ionic liquid-surfactant-salt-water) in extraction of proteins, amino acids, antibiotics and other small molecules in herbs were reviewed. ATPS extraction combining with other technologies were summarized, and the prospect of ATPSs was also discussed.
-
Keywords:
- aqueous two-phase extraction /
- polymer /
- organic solvent /
- ionic liquid /
- hyphenated technique
-
[1] Albertsson P A.Chromatography and partition of cells and cell fragments[J].Nature, 1956, 177 (4513) :771-774.
[2] 刘磊磊, 李秀娜, 赵帅.双水相萃取在中药活性成分提取分离中的应用进展[J].中草药, 2015, 46 (5) :766-773. [3] Xavier L, Freire M S, Vidal-Tato I.Aqueous two-phase systems for the extraction of phenolic compounds from eucalyptus (Eucalyptus globulus) wood industrial wastes[J].Journal of Chemical Technology&Biotechnology, 2014, 89 (11) :1772-1778.
[4] Lakshmi M C, Madhusudhan M C, Raghavarao K S M S.Extraction and purification of lipoxygenase from soybean using aqueous two-phase system[J].Food and Bioprocess Technology, 2009, 5 (1) :193-199.
[5] 王凤薇, 王道武, 郭青锋, 等.双水相萃取黄连生物碱[J].精细化工, 2015 (6) :642-645. [6] Pimentel M C B, Araujo A I, Figueiredo Z M B, et al.Aqueou two-phase system for citrinin extraction from fermentation broth[J].Separation and Purification Technology, 2013, 110:158-163.
[7] 孙亚莉, 张喜峰.双水相萃取分离红豆蛋白质[J].中国酿造, 2013 (6) :114-118. [8] Yuzugullu Y, Duman Y A.Aqueous two-phase (PEG4000Na2SO4) extraction and characterization of an acid invertase from potato tuber (Solanum tuberosum) [J].Preparative Biochemistry&Biotechnology, 2015, 45 (7) :696-711.
[9] Da Silva R M M, Coimbra J S D R, Da Silva C A, et al.Green extraction by aqueous two-phase systems of porcine pancreati and snake venom phospholipase A2[J].Separation and Purification Technology, 2015, 141:25-30.
[10] Wu X T, Li G X, Yang H L, et al.Study on extraction and separation of butyric acid from clostridium tyrobutyricum fermentation broth in PEG/Na2SO4aqueous two-phase system[J].Fluid Phase Equilibria, 2015, 403:36-42.
[11] 郑晓杰, 叶兴乾, 吴祥庭.苦苣皂甙的PEG/Mg SO4双水相萃取优化及其抗氧化评价[J].中国食品学报, 2015 (6) :110-116. [12] Yang X, Zhang S, Yu W, et al.Ionic liquid-anioni surfactant based aqueous two-phase extraction for determination of antibiotics in honey by high-performance liquid chromatography[J].Talanta, 2014, 124:1-6.
[13] Jiang B, Feng Z B, Liu C H, et al.J.Extraction and purification of wheat-esterase using aqueous two-phase system of ionic liquid and salt[J].Journal of Food Science and Technology, 2015, 52 (5) :2878-2885.
[14] Reis I A O, Santos S B, Pereira F D S, et al.Extraction and recovery of rutin from acerola waste using alcohol-salt-based aqueous two-phase systems[J].Separation Science and Technology, 2014, 49 (5) :656-663.
[15] Jiang X M, Lu Y M, Tan C P, et al.Combination of aqueou two-phase extraction and cation-exchange chromatography:new strategies for separation and purification of alliin from garli powder[J].Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 2014, 957:60-67.
[16] Tan Z J, Li F F, Xu X L, et al.Simultaneous extraction and purification of aloe polysaccharides and proteins using ionic liquid based aqueous two-phase system coupled with dialysis membrane[J].Desalination, 2012, 286:389-393.
[17] Reschke T, Brandenbusch C, Sadowski G.Modeling aqueous two-phase systems:II.Inorganic salts and polyether homo-and copolymers as ATPS former[J].Fluid Phase Equilibria, 2014, 375:306-315.
[18] Reis I A O, Santos S B, Santos L A, et al.Increased significance of food wastes:Selective recovery of added-value compounds[J].Food Chemistry, 2012, 135 (4) :2453-2461.
[19] Berton P, Monasterio R P, Wuilloud R G.Selective extraction and determination of vitamin B-12 in urine by ionic liquid-based aqueous two-phase system prior to high-performance liquid chromatography[J].Talanta, 2012, 97:521-526.
[20] Wang Y, Han J, Liu J, et al.Liquid-liquid equilibrium phase behavior of iminazolium-based ionic liquid aqueous two-phase systems composed of 1-alkyl-3-methyl imidazolium tetrafluoroborate and different electrolytes Zn SO4, Mg SO4and Li2SO4at 298.15 K:Experimental and correlation[J].Thermochimica Acta, 2013, 557:68-76.
[21] Wang X H, Wei X L, Liu J, et al.Study on the aqueous twophase systems composed of surfactant, ionic liquid and water[J].Fluid Phase Equilibria, 2013, 347:1-7.
[22] Sarubbo L A, Oliveira L A, Porto A L F, et al.New aqueous two-phase system based on cashew-nut tree gum and poly (ethylene glycol) [J].Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2000, 743 (1-2) :79-84.
[23] Chen X Q, Dong Q L, Yu J G, et al.Extraction of Tryptophan enantiomers by aqueous two-phase systems of ethanol and (NH4) 2SO4[J].Journal of Chemical Technology&Biotechnology, 2013, 88 (8) :1545-1550.
[24] Dembczynski R, Bialas W, Jankowski T.Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4aqueous two-phase system[J].Biochemical Engineering Journal, 2010, 51 (1-2) :24-31.
[25] Wu Y C, Wang Y, Zhang W L, et al.Extraction and preliminary purification of anthocyanins from grape juice in aqueous two-phase system[J].Separation and Purification Technology, 2014, 124:170-178.
[26] He Y Z, Wang B, Zhuang Y, et al.Study on separation and purification of resveratrol in wine grape residue with aqueous two phase extraction method[J].Advanced Materials Research, 2012, 550-553:1743-1746.
[27] Zhao P P, Lu Y M, Tan C P, et al.Aqueous two-phase extraction combined with chromatography:new strategies for preparative separation and purification of capsaicin from capsicum oleoresin[J].Applied Biochemistry and Biotechnology, 2015, 175 (2) :1018-1034.
[28] Tan Z J, Wang C Y, Yi Y J, et al.Extraction and purification of chlorogenic acid from ramie (Boehmeria nivea L.Gaud) leaf using an ethanol/salt aqueous two-phase system[J].Separation and Purification Technology, 2014, 132:396-400.
[29] Zhang W, Zhu D, Fan H J, et al.Simultaneous extraction and purification of alkaloids from Sophora flavescens Ait.by microwave-assisted aqueous two-phase extraction with ethanol/ammonia sulfate system[J].Separation and Purification Technology, 2015, 141:113-123.
[30] Tan Z J, Li F F, Xu X L.Extraction and purification of anthraquinones derivatives from Aloe vera L.using alcohol/salt aqueous two-phase system[J].Bioprocess and Biosystems Engineering, 2013, 36 (8) :1105-1113.
[31] Han J, Hu S P, Wang Y.Extraction of oxytetracycline hydrochloride in aqueous two-phase system of acetone and ammonium sulfate[J].Journal of the Chemical Society of Pakistan, 2013, 35 (1) :11-16.
[32] Gu B H, Zheng P, Yan Q, et al.Aqueous two-phase system:An alternative process for recovery of succinic acid from fermentation broth[J].Separation and Purification Technology, 2014, 138:47-54.
[33] Huang S Y, Wang Y Z, Zhou Y G, et al.Choline-like ionic liquid-based aqueous two-phase extraction of selected proteins[J].Analytical Methods, 2013, 5 (13) :3395.
[34] Zeng Q, Wang Y Z, Li N, et al.Extraction of proteins with ionic liquid aqueous two-phase system based on guanidine ionic liquid[J].Talanta, 2013, 116:409-416.
[35] Chen J, Wang Y Z, Zeng Q, et al.Partition of proteins with extraction in aqueous two-phase system by hydroxyl ammoniumbased ionic liquid[J].Analytical Methods, 2014, 6 (12) :4067.
[36] Ventura S P M, De Barros R L F, De Pinho Barbosa J M, et al.Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems[J].Green Chemistry, 2012, 14 (3) :734.
[37] Shao M Y, Zhang X L, Li N, et al.Ionic liquid-based aqueous two-phase system extraction of sulfonamides in milk[J].Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 2014, 961:5-12.
[38] Cláudio A F M, Ferreira A M, Freire C S R, et al.Optimization of the gallic acid extraction using ionic-liquidbased aqueous two-phase systems[J].Separation and Purification Technology, 2012, 97:142-149.
[39] Yu W, Liu Z L, Li Q, et al.Determination of Sudan I-IV in candy using ionic liquid/anionic surfactant aqueous two-phase extraction coupled with high-performance liquid chromatography[J].Food Chemistry, 2015, 173:815-820.
[40] Almeida M R, Passos H, Pereira M M, et al.Ionic liquids as additives to enhance the extraction of antioxidants in aqueous two-phase systems[J].Separation and Purification Technology, 2014, 128:1-10.
[41] De Brito Cardoso G, Souza I N, Pereira M M, et al.Aqueous two-phase systems formed by biocompatible and biodegradable polysaccharides and acetonitrile[J].Separation and Purification Technology, 2014, 136:74-80.
[42] Wu Q, Lin D Q, Yao S J.Evaluation of poly (ethylene glycol) /hydroxypropyl starch aqueous two-phase system for immunoglobulin G extraction[J].Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 2013, 928:106-112.
[43] Amid M, Manap M Y, Hussin M, et al.A novel aqueous two phase system composed of surfactant and xylitol for the purification of lipase from pumpkin (cucurbita moschata) seeds and recycling of phase components[J].Molecules, 2015, 20 (6) :11184-11201.
[44] Ma F Y, Gu C B, Li C Y, et al.Microwave-assisted aqueous two-phase extraction of isoflavonoids from Dalbergia odorifera T.Chen leaves[J].Separation and Purification Technology, 2013, 115:136-144.
[45] Dang Y Y, Zhang H, Xiu Z L.Microwave-assisted aqueous two-phase extraction of phenolics from grape (Vitis vinifera) seed[J].Journal of Chemical Technology&Biotechnology, 2014, 89 (10) :1576-1581.
[46] Guo T, Su D, Huang Y, et al.Ultrasound-assisted aqueous two-phase system for extraction and enrichment of zanthoxylum armatum lignans[J].Molecules, 2015, 20 (8) :15273-15286.
[47] 马艺丹, 刘红, 廖小伟, 等.神秘果种子多酚超声双水相复合提取工艺及其抗氧化活性[J].食品与机械, 2015 (6) :173-178. [48] 陈钢, 李栋林, 史建鑫, 等.响应面实验优化超声耦合双水相体系提取茶多酚工艺[J].食品科学, 2016 (6) :95-100. [49] 李妍, 李锋, 刘朝兵, 等.双水相辅助超声提取茄肉总黄酮及抗氧化性[J].应用化工, 2016 (1) :107-110. [50] Bi P Y, Chang L, Mu Y L, et al.Separation and concentration of baicalin from Scutellaria Baicalensis Georgi extract by aqueous two-phase flotation[J].Separation and Purification Technology, 2013, 116:454-457.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
