Optimization of preparation process of buffalo milk active peptides microspheres by piercing method and in vitro releasing behavior of the microcapsules
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摘要: 为了减少胃肠道对抗氧化活性肽的分解并使其具有肠道缓释效果,以海藻酸钠、壳聚糖为壁材,用锐孔法制备水牛乳活性肽微胶囊。通过响应面法优化了海藻酸钠溶液浓度、壳聚糖溶液浓度以及Ca2+浓度的工艺条件,并对制备的微胶囊进行扫描电镜观察以及体外释放检测。结果表明:制备载水牛乳活性肽微胶囊的优化工艺为:海藻酸钠、壳聚糖和CaCl2溶液的浓度分别为1.28%、1.51%和2.20%（w/v）,在此工艺条件下,活性肽的包埋率可达95.20%。通过扫描电镜发现,海藻酸钠-壳聚糖复合壁材载肽结构完整紧实。体外缓释实验表明,样品在人工胃液（pH=2.0）中释放量为7.98%,在人工肠液（pH=6.8）中作用12 h后的相对累计释放量为89.41%,样品表现出耐酸和良好的模拟肠道环境缓释效果,制备的水牛乳活性肽微胶囊能够较好的保护目标肽、提高稳定性、耐酸和缓释效果。Abstract: In order to reduce the decomposition of the antioxidant peptide in the gastrointestinal tract and to have a sustained release behavior, encapsulated buffalo milk antioxidant peptides were prepared by piercing method using sodium alginate and chitosan as the shell. Based on peptides loading efficacy, and determined by one-factor experimental results, operating parameters as sodium alginate concentration, chitosan concentration and calcium chloride concentration were optimized by response surface methodology.The prepared microspheres were subjected by scanning electron microscopy and researched on its in vitro releasing behavior.The results showed that the optimal operating parameters were 1.28%, 1.51%, and 2.20% ( w/v) for sodium alginate concentration, chitosan concentration and calcium chloride, respectively. Upon the optimal conditions, the experiment led to an encapsulated efficacy of 95.20%. Scanning electron microscopy showed that the alginate-chitosan microcapsules had compact structure.The in vitro release studies showed that the release rate was only 7.98% in the simulated gastric buffer ( pH = 2.0) , and 89.41% in simulated intestinal buffer ( p H = 6.8) . It showed that the sample had an acid-resistant and good slow-release behavior in simulated intestinal buffer. These results conclusively suggested that encapsulated buffalo mike antioxidant peptides by this process can protect the peptides, and improve its stability, acid resistance and sustained release behavior.
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