Effect of postharvest methionine treatment on blue mould and reactive oxygen metabolism in peach fruit
-
摘要: 以桃果实为材料,研究了采后不同浓度蛋氨酸处理对损伤接种桃果实扩展青霉（Penicillium expansum）病斑扩展的影响,根据病斑直径的大小筛选出能够有效抑制桃果实青霉病的最佳浓度,并以最佳浓度处理桃果实分析对活性氧代谢的影响。结果表明,15 mmol/L蛋氨酸对P.expansum的抑制效果最好,并且处理显著（p≤0.05）提高了桃果实中抗坏血酸（AsA）和还原型谷胱甘肽（GSH）含量;谷胱甘肽还原酶（GR）活性、抗坏血酸过氧化物酶（APX）活性和过氧化氢酶（CAT）活性也显著提高,同时提高了H2O2含量。由此表明,采后蛋氨酸处理可以通过调节桃果实体内活性氧代谢来提高果实对青霉病的抗性。Abstract: Peach fruit was used as materials to study the effects of postharvest methionine treatment on lesion development of peach fruit inoculated with Penicillium expansum.The optimum concentration of methionine was selected according to the lesion diameter,and then to analyzing the effect of methionine on the reactive oxygen metabolism in peach fruit.The results indicated that methionine at the concentration of 15 mmol/L effectively inhibited lesion development of peach fruit inoculated with P.expansum.The results also indicated that methionine treatment significantly( p≤0.05) increased the content of ascorbic acid(AsA) and glutathione(GSH),enhanced the activity of glutathione reductase(GR),ascorbate peroxidase(APX) and catalase(CAT) and the content of H_2O_2 in peach fruit. The results suggest that postharvest methionine treatment could improve the resistance of peach fruit against P.expansum by regulating the reactive oxygen metabolism.
-
Keywords:
- methionine /
- peach /
- reactive oxygen species /
- blue mould /
- disease resistance
-
[1] Sasaki F F,Cerqueira T S,Sestari I,et al.Woolliness control and pectin solubilization of‘Dourado’peach after heat shock treatment[J].Acta Horticulturae,2010,877:539-542.
[2] Liu H X,Jiang W B,Bi Y,et al.Postharvest BTH treatment induces resistance of peach(Prunus persica L.cv.Jiubao)fruit to infection by Penicillium expansum and enhances activity of fruit defense mechanisms[J].Postharvest Biology and Technology,2005,35(3):263-269.
[3] Tripathi P,Dubey N K.Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables[J].Postharvest Biology and Technology,2004,32(3):235-245.
[4] Droby S,Wisniewski M,Macarisin D,et al.Twenty years of postharvest biocontrol research:Is it time for a new paradigm?[J]Postharvest Biology and Technology,2009,52(2):137-145.
[5] Kang N J.Inhibition of powdery mildew development and activation of antioxidant enzymes by induction of oxidative stress with foliar application of a mixture of riboflavin and methionine in cucumber[J].Scientia Horticulturae,2008,118(3):181-188.
[6] Sarosh B R,Sivaramakrishnan S,Shetty H S.Elicitation of defense related enzymes and resistance by L-methionine in pearl millet against downy mildew disease caused by Sclerospora graminicola[J].Plant Physiology and Biochemistry,2005,43(8):808-815.
[7] Boubakri H,Wahab M A,Chong J L,et al.Methionine elicits H2O2generation and defense gene expression in grapevine and reduces Plasmopara viticola infection[J].Journal of Plant Physiology,2013,170(18):1561-1568.
[8] 周春华,刘红霞,韦军.活性氧与果实成熟衰老[J].上海交通大学学报(农业科学版),2002,20(1):77-84. [9] 邓惠文,毕阳,葛永红,等.采后BTH处理及粉红单端孢(Trichothecium roseum)挑战接种对厚皮甜瓜果实苯丙烷代谢活性的诱导[J].食品工业科技,2013,34(1):323-326. [10] Ge Y H,Deng H W,Bi Y,et al.Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon(Cucumis melo L.)fruit[J].Postharvest Biology and Technology,2015(99):160-167.
[11] Ren Y L,Wang Y F,Bi Y,et al.Postharvest BTH treatment induced disease resistance and enhanced reactive oxygen species metabolism in muskmelon(Cucumis melo L.)fruit[J].European Food Research and Technology,2012,234(6):963-971.
[12] Nakano Y,Asada K.Hydrogen peroxidase is scavenged by ascorbate-specific peroxidase in spinach chloropasts[J].Plant Cell Physiology,1981,22(5):867-880.
[13] Halliwell B,Foyer C H.Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromotography[J].Planta,1978,139(1):9-17.
[14] Turcsányi E,Lyons T,Plchl M,et al.Does ascorbate in the mesophyll cell walls form the first line of defence against ozone?Testing the concept using broad bean(Vicia faba L.)[J].Journal of Experimental Botany,2000,346(51):901-910.
[15] 葛永红,毕阳,李永才,等.苯并噻重氮(ASM)对果蔬采后抗病性的诱导及机理[J].中国农业科学,2012,45(16):3357-3362. [16] 马春花,张敏,李明军,等.外源As A对苹果果实采后衰老及As A-GSH循环的影响[J].云南农业大学学报:自然科学版,2012,27(3):384-390. [17] 李灿婴,葛永红,朱丹实,等.采后ASM处理对苹果果实抗坏血酸-谷胱甘肽循环系统的影响[J].食品工业科技,2014,35(23):333-337. [18] Zeng K F,Cao J K,Jiang W B.Enhancing disease resistance in harvested mango(Mangifera indica L.cv.‘Matisu’)fruit by salicylic acid[J].Journal of the Science of Food and Agriculture,2006,86(5):694-698.
[19] Cao S F,Zheng Y H,Yang Z F,et al.Effect of methyl jasmonate on the inhibition of Colletotrichum acutatum infection in loquat fruit and the possible mechanisms[J].Postharvest Biology and Technology,2008,49(2):301-307.
[20] Ge Y H,Deng H W,Bi Y,et al.Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon(Cucumis melo L.)fruit[J].Postharvest Biology and Technology,2015,99(1):160-167.
[21] Ge Y H,Deng H W,Bi Y,et al.The role of reactive oxygen species in ASM-induced disease resistance in apple fruit[M].In:D.Prusky;M.L.Gullino(eds)Postharvest Pathology,Springer,2014,39-52.
[22] Noctor G,Foyer C H.Ascorbate and glutathione:keeping active oxygen under control[J].Annual Review of Plant Biology,2003,49(4):249-279
[23] Shigenaga T,Yamauchi N,Funamoto Y,et al.Effects of heat treatment on an ascorbate-glutathione cycle in stored broccoli(Brassica oleracea L.)florets[J].Postharvest Biology and Technology,2005,38(2):152-159.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
