Identification of Lepidium meyenii(Maca)and its derived products with PCR method
-
摘要: 本研究通过玛卡的defensin基因序列,设计了玛卡的特异性引物,建立了玛卡及其制品中玛卡源性成分检测的一种新方法。通过物种特异性测试,所设计的引物能够特异性扩增玛卡基因,并且,DNA浓度的检测灵敏度达到0.1 ng/μL以上,重量灵敏度达到0.1%以上。并结合芜菁源性成分基因检测方法对市场随机抽取的21份玛卡及其制品进行检测,21份样品均检出了玛卡源性成分而未检出芜菁源性成分。本研究所建立的PCR检测方法快速,准确,高效,适用于市场上玛卡及其制品真伪的快速鉴别。Abstract: According to the defensin gene sequences of Lepidium meyenii,a pair of specific primers of Lepidium meyenii was designed and a novel method for detection of Lepidium meyenii-origin genes was established.Based on the species-specificity and sensitivity testing,the designed Lepidium meyenii primer was of good species-specific and the novel method was of high sensitivity,with 0.1 ng/mL of DNA sensitivity and 0.1% of weight sensitivity,respectively. Using this method combining with the detection of rappini-origin genes,21 samples of Lepidium meyenii and its manufactured goods from the market were randomly measured.The results showed that all the samples contained the Lepidium meyenii-origin genes,but not the rappiniorigin genes. The PCR method established in this study was rapid,accurate,efficient and suitable for the identification of Lepidium meyenii and its manufactured goods from the market.
-
Keywords:
- maca; /
- Lepidium meyenii; /
- polymerase chain reaction; /
- identification;
-
[1] 余龙江,金文闻,李为,等.南美植物玛咖的研究进展[J].中草药,2003,34(2):197-199. [2] Cicero A F,Bandieri E,Arietti R.Lepidium meyenii Walp.Improves sexual behaviour in male rats independently from its action on spontaneous locomotor acticity[J].Journal of Ethnopharmacology,2001,75:225-229.
[3] 余龙江,金文闻.玛咖(Lepidium meyenii)干粉的营养成分及抗疲劳作用研究[J].食品科学,2004,25(2):164-166. [4] 曹东,薛润光,顾鉴秋.丽江玛咖不同提取物对小鼠性行为及性激素的影响[J].云南中医中药杂志,2012,33(9):53-54. [5] 孙晓东,唐辉,杜萍,等.丽江玛咖的营养成分分析及多糖体外的抗氧化作用[J].光谱实验室,2013,30(5):2365-2371. [6] Gonzales G F,Leiva-Revilla J,Rubio J,et al.Effect of red maca(Lepidium meyenii)on prostate zinc levels in rats with testosterone-induced prostatic hyperplasia[J].Andrologia,2012,44(Suppl 1):362-369.
[7] Kuang Y F,Chen Y H.Induction of apotosis a non-samll cell human lung cancer cell line by isothiocyanates is associated with P53 and P51[J].Food and chemical Tocicology,2004,42:1711-1718.
[8] Chacon G.Estudio fitoquimico de Lepidium meyenii Walp[M].Lima:Universidad mayor de San Marcos,1962.
[9] Jin W W,Xiong Y,Yu L J.Identification and quality evaluation of Lepidium meyenii.(Maca)based on gas chromatographic analysis of its essential oils from roots[J].Agricultural science and technology,2006,7(3):2-9.
[10] 李雪芳,邱斌,蒲星宇.玛咖与混淆品蔓菁的比较研究[J].中药材,2015,38(8):1643-1645. [11] 黄宇锋,罗海英,罗东辉,等.PCR方法快速鉴别食品中肉的种类[J].现代食品科技,2013,29(7):722-724. [12] 罗家琴,王加启,卜登攀,等.饲料中牛、羊、猪、鸡源性成分的PCR检测方法及其应用[J].中国农业科学,2008,41(7):2112-2119. [13] 覃芳芳,王德莲,冼燕萍,等.鱼翅类食品中鲨鱼成分PCR鉴定方法研究[J].现代食品科技,2014,30(4):274-278.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
