Butyl hydroxyanisole induced astaxanthin accumulation in Haematococcus pluvialis LUGU
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摘要: 利用不同诱导条件促进雨生红球藻Haematococcus pluvialis积累虾青素已成为虾青素生产和研究的热点。实验研究了不同质量浓度的茴香醚(BHA)对胁迫培养条件下雨生红球藻Haematococcus pluvialis LUGU生长和虾青素积累的影响。结果表明,2 mg/L的BHA能够显著提高虾青素的含量,最大含量可达29.03 mg/g,比对照组(14.30 mg/g)提高到2.03倍。而在相同的茴香醚诱导条件下,初始藻细胞的不同生长状态对细胞的生长及虾青素的合成也有很大的影响:处于对数生长期后期(14 d)的细胞更有利于虾青素的积累,其最大虾青素含量达到29.3 mg/g,分别比前期、中期和稳定期提高到2.9、1.01和1.72倍。实验表明茴香醚是虾青素诱导的有效诱导子,本研究也可为两阶段法诱导生产虾青素提供依据。Abstract: Haematococcus pluvialis is a potential source for astaxanthin accumulation under the unfavorable condition,which has gained more and more attention in recent years.In this paper,different concentration of Butyl hydroxyanisole( BHA) were studied for their affection the production of astaxanthin in Haematococcus pluvialis LUGU.The results indicated that the 2 mg / L BHA was optimal for astaxanthin production with 29.03 mg / g,which was 2.03 times that of the control( 14.30 mg / g).Moreover,the accumulation of astaxanthin in the algae cells,which were in the late- exponential growth phase( 14 d),reached the highest production of 29.3 mg / g( 2.9,1.01 and 1.72 times than that of other cells). The result showed the BHA was an effective inductor for astaxanthin accumulation and the different condition of algae cells was important for astaxanthin production with two- stage strategy.
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