Application of membrane separation on preliminary purification of resveratrol from extract of peanut root
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摘要: 为利用花生根中白藜芦醇资源,将膜分离应用于花生根提取液中白藜芦醇的初级纯化,以膜通量和膜渗透性、白藜芦醇和水溶性糖截留率为评价指标,考察由超滤和纳滤组成膜分离系统的工艺参数与影响因素。结果如下:超滤膜UE030和纳滤膜NF4适宜于花生根提取液中白藜芦醇的分离纯化;提取液p H会显著影响纳滤膜对白藜芦醇的截留能力;操作压力会显著影响纳滤膜通量和渗透性,但对白藜芦醇和水溶性糖的截留率无显著影响;最佳膜分离工艺为花生根提取液经过1 MPa、400 r/min、UE030超滤后,透过液调节至p H6.0,采用3 MPa,400 r/min搅拌,NF4纳滤后,水溶性糖浓度从(1.1832±0.0718)mg/m L下降到(0.0475±0.0007)mg/m L,白藜芦醇浓度为(4.4730±0.0129)μg/m L,回收率为55.84%±0.16%。以上结果证明,膜分离可用于白藜芦醇的初级分离纯化,促进从花生根中制备白藜芦醇的工业化发展。Abstract: To utilize resveratrol resource in peanut roots,membrane separation had been employed in the preliminary separation of resveratrol in crude extract of peanut roots.Process parameters and influencing factors of a sequential membrane separation system consisting of ultrafiltration and nanofiltration were investigated through comprehensively considering performances of permeate flux,permeability,rejections of resveratrol and soluble sugar. Results showed that ultrafiltration membrane UE030 and nanofiltration membrane NF4 were suitable to separate resveratrol from crude extract of peanut root.p H of extract of peanut root had a significant influence to the performance of nanofiltration membrane on rejection of resveratrol. Operational pressure would remarkably affect permeate flux and permeability of nanofiltration membrane,but insignificantly affect rejections of resveratrol and soluble sugar.The optimal process of membrane separation was as follows: crude extract of peanut root was firstly ultrafiltrated by UE030 under 1 MPa,400 r / min,then the permeate of ultrafiltration was nanofiltrated by NF4 under3 MPa,400 r / min,finally the concentration of soluble sugar decreased from( 1.1832 ± 0.0718) mg / m L to( 0.0475 ±0.0007) mg / m L,the concentration and recovery of resveratrol were( 4.4730 ± 0.0129) ug / m L and( 55.84% ±0.16%),respectively. It could be concluded that membrane separation manages to preliminarily separate and purify resveratrol from peanut roots,which promoted the development of commercial production of resveratrol from peanut roots.
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Keywords:
- ultrafiltration /
- nanofiltration /
- peanut root /
- resveratrol
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