Extraction flavonoids from Sambucus williamsii Hance leaves and evaluation of antioxidant activities
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摘要: 在单因素实验的基础上,采用响应面法对接骨木叶片总黄酮的超声辅助提取工艺进行了优化,并对接骨木叶片总黄酮乙醇提取物的乙酸乙酯、氯仿、正丁醇、水四种不同极性溶剂分级萃取组分的抗氧化活性进行了分析。结果表明:超声辅助提取接骨木叶片总黄酮的最佳工艺条件为:乙醇浓度70%,料液比1∶40,提取温度70℃,提取时间60 min,在此条件下,总黄酮最高提取得率可达18.67%;乙酸乙酯、氯仿、正丁醇、水四种极性萃取组分中黄酮相对含量分别为54.3%、28.22%、22.01%、9.817%,且四种组分对DPPH自由基和ABTS+自由基均有一定的清除活性,但均以乙酸乙酯萃取组分的清除活性最高,说明接骨木叶片乙酸乙酯萃取组分富含天然抗氧化剂,具有潜在的开发价值。Abstract: On the basis of single factor test,response surface method was adopted to optimize the ethanol extraction process with ultrasonic assistance for total flavonoids of Sambucus williamsii Hance leaves. Moreover,analysis on antioxidant activity of different fractions extracted by solvents with different polarity from the ethanol extract had been conducted for Sambucus williamsii Hance leaves,such as,ethyl acetate,chloroform and butanol.Result showed that,the best process condition of extracting total flavonoids of elderberry leaves with ultrasonic assistance were as follows: ethanol concentration was 70%,solid- liquid ration was 1 ∶ 40,extraction temperature was 70 ℃,extraction time was 60 min. Under this condition,the highest extraction rate of total flavonoids could reach up to 18.67%. The four polarity extraction constituents of Sambucus williamsii Hance leaves,ethyl acetate,chloroform,butanol and water,relative contents of flavonoid from extracts were 54.3%,28.22%,22.01%,9.817%,and they had a certain of scavenging activity against DPPH and ABTS+free radicals. In fact,the scavenging activity of ethyl acetate was the highest,which indicated that,the extraction of ethyl acetate was the optimized part to separate natural antioxidant from Sambucus williamsii Hance leaves.
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Keywords:
- Sambucus williamsii Hance /
- flavonoids /
- extraction /
- antioxidant
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