Study on tolerance characteristics of the gastrointestinal tract and cholesterol-lowering effects of Lactobacillus plantarum Zhang-LL
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摘要: 利用从发酵米粉中筛选出的一株植物乳杆菌Zhang-LL探讨其胃肠道耐受性及体外降胆固醇功效。本实验分别在p H1.53.0、胆盐浓度0.1%0.5%的条件下模拟人体胃肠道环境,采用活菌计数法检测菌株Zhang-LL耐胃肠道逆环境特性及邻苯二甲醛法检测降胆固醇效果。结果表明:初始菌数为1×107CFU/m L的植物乳杆菌Zhang-LL在p H1.53.0的环境中3 h后,活菌数仍可达106CFU/m L;在胆盐浓度为0.10%0.50%环境中8 h,活菌数可达105107CFU/m L;体外胆固醇降低率在20.69%35.68%范围。研究表明植物乳杆菌Zhang-LL具有较强的耐胃肠道逆环境特性及良好的降胆固醇能力,为开发研制降胆固醇功能食品及微生态制剂提供了实验基础。Abstract: The tolerance characteristics of the gastrointestinal tract and cholesterol- lowering effects of Lactobacillus plantarum Zhang- LL isolated from fermented rice noodles in Bama,Guangxi Province were investigated. Viable count was firstly used in this study to detect the tolerance characteristics under simulated gastrointestinal tract environment p H1.5~ 3.0. Additionally,bacterial growth was evaluated in the presence of 0.10% ~ 0.50% bile salt.O- phthalaldehyde was used to detect its cholesterol- lowering capability. The results showed that Lactobacillus plantarum Zhang-LL's number of viable bacteria was still up to 106 CFU / m L after 3 h under p H1.5~ 3.0,whose initial bacterial count was 1 × 107 CFU / m L. And being cultured for 8 h in the MRS- broth( 0.10% ~ 0.50% bile salt),the viable count reached nearly to 105~ 107 CFU / m L. A cholesterol removal rate ranging from 20.69% to 35.68% was observed under varying conditions.It showed that Lactobacillus plantarum Zhang- LL had a strong capability of anti gastric intestinal reverse environment and good cholesterol lowering ability,which provided the experimental basis for the development of cholesterol- lowering functional food and micro ecological preparation.
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Keywords:
- Lactobacillus plantarum /
- cholesterol-lowering /
- gastrointestinal tract /
- tolerance
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[1] Steomberg D,Parthasarthy S,Carew TE,et al.Beyond cholesterol modification of low density lipoprotein that increase its atherogenicity[J].The New England Journal of Medicine,1989,320(14):915-924.
[2] 隋在云,盛国良,李英霞,等.复方降脂软胶囊对高脂血症大鼠血液流变学的影响[J].河南中医,2009,29(4):353-354. [3] Perdigon G.Systemic augmentation of the immune response in mice by feeding fermentedmilks with Lactobacillus casei and Lactobacillus acidophilus.[J].Immunology,1988:63(1):17-23.
[4] Mann GV.Study of surfactant and cholesteremia in the Maasai[J].The American Journal of Clinical Nutrition,1974,27(5):464-469.
[5] Jones M,Martoni C,Parent M,et al.Cholesterol-lowering efficacy of a microencapsulated bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 yoghurt formulation in hypercholesterolaemic adults[J].Br J Nutr,2012,107(10):1505-1513.
[6] Rajesh Kumar,Sunita Grover,Virender Kumar Batish.Hypocholesterolaemic effect of dietary inclusion of two putative probiotic bile salt hydrolase-producing Lactobacillus plantarum strains in Sprague-Dawley rats[J].British Journal of Nutrition,2011,105(4):561-573.
[7] Anderson JW,Gilliland SE.Effect of Fermented Milk Containing Lactobacillus acidophilus L1 on Serum Cholesterol in Hypercholesterolemic Humans[J].Journal of the American College of Nutrition,1999,18(1):43-50.
[8] Nguyen TD,Kang JH,Lee MS.Characterization of Lactobacillus plantarum PH04,a potential probiotic bacterium with cholesterol-lowering effects[J].International Journal of Food Microbiology,2007,113(3):358-361.
[9] 谢宁.两株乳酸杆菌对高脂饮食大鼠胆固醇影响及相关机制研究[D].长沙:中南大学,2011. [10] 李婷婷.降胆固醇益生乳酸菌的筛选及其在大鼠体内的应用研究[D].哈尔滨:东北农业大学,2013. [11] 刘慧.现代食品微生物学实验技术[M].北京:中国轻工业出版社,2006:143-147,263. [12] Rudel L L,Morris M D.Determination of cholesterol using o-phthalaldehyde[J].Jorunal of Lipid Research,1973,14:364-366.
[13] Psomas E I,Fletouris D J,Litopoulou-Tzanetaki E,et al.Assimilation of cholesterol by yeast strains isolated from infant feces and feta cheese[J].Journal of Dairy Science,2003,86(86):3416-3422.
[14] 赖文,刘书亮,张倩颖,等.降胆固醇乳酸菌鉴定及其在体外模拟胃肠环境中抗性研究[J].中国酿造,2011(3):90-93. [15] Gilliland SE,Nelson CR,Maxwell C.Assimilation of cholesterol by Lactobacillus acidophilus[J].Applied&Environmental Microbiology,1985,49(2):377-381.
[16] DIA Pereira,GR Gibson.Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut[J].Applied and Environmental Microbiology,2002,68(9):4689-4693.
[17] 靳志强,王延祥,李平兰,等.植物乳杆菌耐酸耐胆盐的体外实验及其降胆固醇作用[J].中国食品学报,2009(9):24-28. [18] Shimada K,Bricknell KS,Finegold SM.Deconjugation of bile acid by intestinal bacteria:a review of literature and additional studies[J].The Journal of Infectious Diseases,1969,119(3):273-281.
[19] Razin S,Tully JG.Cholesterol Requirement of Mycoolasmas[J].Journal of Bacteriology,1970,102(2):306-310.
[20] ZHAO Ruixiang,SUN Junliang,MO Haizhen,et al.Analysis of functional properties of Lactobacillus acidophilus[J].World Journal of Microbiology&Biotechnology,2007,23(2):195-200.
[21] 李常营,卢晓霆,于志会,等.酸菜来源植物乳杆菌S4-5的降胆固醇作用[J].食品科学,2011(7):69-72.
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