Optimization of medium for bacterial strain of carotenoids degradation
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摘要: 为了提高类胡萝卜素的降解率,对霍氏肠杆菌（Enterobacter hormaechei）A20菌株的发酵培养基成分进行响应面优化。通过单因素实验确定了最佳碳源、氮源和无机盐分别为葡萄糖、混合氮源（酵母膏∶蛋白胨=2∶1）和K2HPO4,再以这3个因素为考察因子,以类胡萝卜素降解率为响应值,设计了3因素3水平的Box-Behnken响应面分析实验,得到降解类胡萝卜素的最佳培养基组成:葡萄糖8 g/L,混合氮源（酵母膏∶蛋白胨=2∶1）14 g/L,K2HPO40.72 g/L。类胡萝卜素降解率从最初的45.7%提高到77.37%,与初始的发酵培养基相比提高了69.30%。说明Box-Behnken实验设计法用于降解类胡萝卜素菌株的培养基优化是可行的,数学模型的预测值与实验观察值相符。
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关键词:
- 类胡萝卜素降解率 /
- 培养基优化 /
- Box-Behnken设计 /
- 响应面
Abstract: In order to improve the degradation rate of carotenoids,optimization of the fermentation medium for Enterobacter hormaechei A20 strain was carried out by response surface analysis.The optimal carbon source,nitrogen source and inorganic salts source for A20 were glucose,a mixed nitrogen source( yeast extract∶ peptone= 2∶ 1) and K2HPO4,respectively.Then three critical factors were selected as response factors,the Box-Behnken response surface analysis test of 3 factors and 3 levels was designed with the degradation rate of carotenoid as the response value.As a result,the optimum fermentation medium was composed of glucose 8 g / L,mixed nitrogen sources( yeast extract∶ peptone = 2 ∶ 1) 14 g / L and K2HPO4 0.72 g / L.Under the situation,carotenoids degradation rate increased from 45.7% to 77.37%,which increased by 69.30% compared with the original medium.The observed responses are in close agreement with the predicted values of the mathematic models,and the Box-Behnken design is suitable for optimize the culture medium of the degradation of carotenoids. -
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