Cloning and gene sequence analysis of pgd S and ggt of poly-γ-glutamate degrading enzymes system
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摘要: 以实验菌株枯草芽孢杆菌Bacillus subtilis 115基因组为模板,通过PCR技术成功克隆到γ-聚谷氨酸降解酶系基因pgd S和ggt,并进行测序,利用Ex PASy-Prot Param tool、Server 3.0 Signal P、TMHMM Server和Tmpred、PSORTB、Predict Protein、Swiss-Model Workspace软件,分别对蛋白质的理化性质、信号肽、跨膜区、亚细胞定位、二级结构、三维结构建模进行了分析和预测。结果表明:pgd S和ggt基因分别含1242个和1764个核苷酸,分别编码414个和588个氨基酸。Pgd S和GGT均为稳定型亲水性蛋白,都存在信号肽,其亚细胞分别定位于胞壁和胞外。GGT在N端存在一个强跨膜区。二级结构分析显示,Pgd S和GGT两种蛋白都以L（环）为主,分别占53.27%和52.47%。通过对γ-PGA降解酶系蛋白结构的分析,为日后有效控制γ-PGA合成及相关研究提供参考和理论依据。
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关键词:
- Bacillus subtilis 115 /
- γ-PGA /
- pgd S基因 /
- ggt基因 /
- 生物信息分析
Abstract: The gene of pgd S and ggt were cloned from Bacillus subtilis 115 genome DNA by PCR. Physical and chemical properties,trans- membrane domains,sub- cellular localization,secondary structures,and three dimensional structure modeling of Pgd S and GGT were analyzed using the software of Ex PASy- Prot Param,Server3.0 Signal P,TMHMM Server and Tmpred,PSORTB,Predict Protein,Swiss- Model Workspace,respectively. The results showed that the pgd S genes contained 1242 nucleotides and code 414 amino acids. The ggt genes contained 1764 nucleotides and code 588 amino acids. By amino acid sequence analysis,there was a strong transmembrane domain in the N side of GGT. Pgd S and GGT were stable hydrophilic proteins,existence signal peptide and its subcellular localization in the cell wall and outside of cell,respectively. The secondary structure of Pgd S and GGT were mainly loop,which accounted for 53.27% and 52.47% respectively.The process of γ- PGA synthesis and related research were effectively controlled through the analysis of the structure characteristics of theγ- PGA degradation enzyme.-
Keywords:
- Bacillus subtilis 115 /
- γ-PGA /
- pgdS /
- ggt /
- biological information analysis
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