Effects of different methods on physico- chemical properties of collagen deprived from Rana chensinensis (Rana dybowskii) skins
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摘要: 以东北林蛙皮为原材料,用乙酸和胃蛋白酶两种方法对其中所含的胶原蛋白进行提取,最终得到两种胶原蛋白:酸溶性胶原蛋白（ASC）和酶溶性胶原蛋白（PSC）,并对这两种胶原蛋白的理化性质及功能特性进行比较研究。结果发现,ASC和PSC在234 nm附近都有强吸收峰,符合胶原蛋白的特征;红外光谱发现ASC在3346.0、2952.0、1662.0、1548.0、1242.0 cm-1有吸收峰,PSC在3322.0、2944.0、1662.0、1551.0、1236.0 cm-1有吸收峰,证明这两种胶原蛋白都存在酰胺A、酰胺B、酰胺Ⅰ、酰胺Ⅱ、酰胺Ⅲ,内部三螺旋结构都保留完整;氨基酸组成表明ASC和PSC都含有18种氨基酸,包括人体所需的8种必需氨基酸,但是组成略有差异;SDS-PAGE电泳结果显示,这两种胶原蛋白都存在β、α1和α2链,符合Ⅰ型胶原蛋白的结构特征;PSC的变性温度较ASC要高,但是保湿率较低,它们的吸湿率和相对溶解度差异不显著（p>0.05）;以上结果表明不同提取方法对东北林蛙皮胶原蛋白的结构、功能特性有一定影响,但是不影响胶原蛋白的品质。Abstract: Rana chensinensis skins as raw materials,two methods,being acid and pepsin respectively,were used to prepare collagen,eventually,getting two collagens: acid- solubilized collagen( ASC) and pepsin- solubilized collagen( PSC),of which physico- chemical properties and functional characteristics were compared and studied.Results showed that there was a strong absorption peak at 234 nm by analyzing UV spectrums,which demonstrated the two kinds of collagens were in accordance with collagens,characteristics.ASC and PSC had absorption peaks at3346.0,2952.0,1662.0,1548.0,1242.0 cm- 1and 3322.0,2944.0,1662.0,1551.0,1236.0 cm- 1respectively by analyzing IR spectrums,which proved the existences of amide A,amide B,amideⅠ,amideⅡ,amide Ⅲ,indicating that triple helical conformation of the two kinds of collagens were reserved completely. According to analyze amino acids compositions,there were 18 kinds of amino acids in ASC and PSC,including 8 kinds of necessary amino acids humans need,of which were a little different between ASC and PSC. SDS-PAGE electrophoresis results showed that both collagen existed β,α1and α2chain,in line with structural features of collagen typeⅠ.Compared with ASC,PSC was higher in denaturation temperature,but was lower in moisturizing efficiency,there were not significant for them in moisture absorption rate and the relative solubility( p < 0.05).These results described above suggested that different extraction methods have certain influence on the structure,functional characteristics of collagen from Rana chensinensis skins,but did not affect the quality of collagen.
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[1] Ogawa M,Portier R J,Moody M W,et al.Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum(Pogonia cromis)and sheepshead sabream(rchosargus probatocephalus)[J].Food Chemistry,2004,88(4):495-501.
[2] Schegg B,Hulsmeier Andreas J,Rutschmann C,et al.Core glycosylation of collagen is initiated by twoβ(1-O)galactosyl transferases[J].Molecular and Cellular Biology,2009,29(4):943-952.
[3] 王碧,王坤余,张廷有,等.一种新型可食性包装材料:胶原蛋白-葡甘聚糖(C-KGM)共混膜[J].中国皮革,2004,33(11):1-3,6. [4] 陈冬英.胶原蛋白与化妆品[J].香料香精化妆品,2001(6):18-19. [5] Wakitani S,Goto T,Young RG,et al.Repair of large fullthickness articular cartilage defects with allograft articular chondrocytes embedded in a collagen gel[J].Tissue Eng,1998,4(4):429-444.
[6] 肖玉良,郑连英,韩俊芬,等.胶原蛋白研究进展[J].泰山医学院学报,2005,26(5):493-501. [7] 傅燕凤,沈月新,杨承刚,等.淡水鱼鱼皮胶原蛋白的提取[J].上海水产大学学报,2004,13(2):146-150. [8] 国家药典委员会.中华人民共和国药典[M].二部.北京:化学工业出版社,2010. [9] 朱瑞霞,施溯筠.冰乙酸和胃蛋白酶依次提取新鲜林蛙皮胶原蛋白[J].延边大学学报:自然科学版,2013,39(3):202-205. [10] 郑淼,周亚丹,赵敏.东北林蛙皮中胶原蛋白含量的测定及提取工艺[J].东北林业大学学报,2008,36(7):81-83. [11] 罗凤莲,夏延斌,欧阳建勋,等.分光光度法测定鸡肉中的羟脯氨酸含量[J].食品科技,2010,35(1):122-124. [12] 李八方,郭鸣,侯虎,等.胡子鲶鱼皮酸溶性胶原蛋白的理化性质研究[J].现代食品科技,2013,29(11):2580-2585. [13] Lin Wang,Qiufang Liang,Tingting Chen,et al.Characterization of collagen from the skin of Amur sturgeon(Acipenser schrenckii)[J].Food Hydrocolloids,2014,38(38):104-109.
[14] 赵岩,侯莹莹,唐国胜,等.柱前衍生RP-HPLC测定淫羊藿中氨基酸含量[J].药物分析杂志,2014,34(8):1412-1416. [15] 庄永亮,赵雪,张朝辉,等.海蜇伞部胶原蛋白的提取及其理化性质[J].食品科学,2009,30(13):89. [16] 施昌松,崔凤玲,张洪广,等.化妆品常用保湿剂保湿吸湿性能研究[J].日用化学品科学,2007,30:25-30. [17] 陶宇,李志皓,侯虎,等.不同方法提取沙海蜇皮胶原蛋白的对比分析[J].食品工业科技,2012,33(14):310-312,326. [18] Phanat Kittiphattanabawon,Soottawat Benjakul,Wonnop Visessanguan,et al.Characterisation of acid-soluble collagen from skin and bone of bigeye snapper(Priacanthus tayenus)[J].Food Chemistry,2005,89:363-372.
[19] 赵苍碧,黄玉东,李艳辉.从牛腱中提取胶原蛋白的研究[J].哈尔滨工业大学学报,2004,36(4):516-519. [20] J H Muyonga,C G B Cole,K G Duodu.Characterisation of acid soluble collagen from skins of young and adult Nile perch(Lates niloticus)[J].Food Chemistry,2004,85(1):81-89.
[21] 刘书成.水产食品加工学[M].郑州:郑州大学出版社,2011. [22] 杨霞,王珊珊,赵芙钗,等.驴皮中胶原蛋白的提取及其特性[J].精细化工,2011,28(9):884-886. [23] 张强,王倩倩,陆剑锋,等.不同方法提取鲢鱼皮胶原蛋白的理化特性比较[J].现代食品科技,2014,30(5):104-110.
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